The anti-endotoxic effects of the KSL-W decapeptide on Escherichia coli O55:B5 and various oral lipopolysaccharides

Background and Objective: Host responses following the recognition of bacterial lipopolysaccharide can range from acute inflammation to septic shock. The aim of this study was to evaluate the ability of the KSL‐W decapeptide to bind to and block the endotoxic effects of lipopolysaccharide. Material and Methods: An enzyme‐linked immunosorbent assay‐based binding assay using fluorescently labeled KSL‐W to detect adsorbed Escherichia coli O55:B5 lipopolysaccharide was employed. A commercially available recombinant Factor C lipopolysaccharide detection assay, hemagglutination of rabbit erythrocytes as well as E‐selectin expression in human umbilical vein endothelial cells were used to assess the anti‐endotoxic effects after KSL‐W exposure to E. coli lipopolysaccharide as well as to oral lipopolysaccharide samples. Results: Lipopolysaccharide‐binding assays using E. coli O55:B5 lipopolysaccharide revealed both a higher maximal binding range (532–713 μm) and a half‐maximum binding concentration (70–185 μm) for the KSL‐W peptide when compared with its analog control. Significant inhibition of E‐selectin expression in human umbilical vein endothelial cells (p