Cloning and characterization of the L15 ribosomal protein gene homologue from the crayfish Orconectes limosus
A cDNA homologue of the large unit rat ribosomal protein L15 was cloned from an epidermal cDNA library of the crayfish Orconectes limosus, being the first crustacean ribosomal protein gene cloned to date. It contains 204 amino acids, deduced from the nucleotide sequence, and reveals 70-76% sequence...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1999-03, Vol.256 (2), p.313-316 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Tom, M Waterman, M R Böcking, D Dauphin-Villemant, C |
| description | A cDNA homologue of the large unit rat ribosomal protein L15 was cloned from an epidermal cDNA library of the crayfish Orconectes limosus, being the first crustacean ribosomal protein gene cloned to date. It contains 204 amino acids, deduced from the nucleotide sequence, and reveals 70-76% sequence identity with other arthropod and vertebrate ribosomal L15s. A single, abundant 0.7-kb mRNA transcript was constitutively expressed and revealed similar expression levels, among various adult crayfish tissues. |
| doi_str_mv | 10.1006/bbrc.1999.0322 |
| format | Article |
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It contains 204 amino acids, deduced from the nucleotide sequence, and reveals 70-76% sequence identity with other arthropod and vertebrate ribosomal L15s. 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| ispartof | Biochemical and biophysical research communications, 1999-03, Vol.256 (2), p.313-316 |
| issn | 0006-291X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Amino Acids - chemistry Amino Acids - genetics Animals Astacoidea - genetics Base Sequence Blotting, Northern Cloning, Molecular Epidermis Freshwater Gene Expression Gene Library Humans Molecular Sequence Data Open Reading Frames - genetics Orconectes limosus Ribosomal Proteins - chemistry Ribosomal Proteins - genetics Sequence Alignment Sequence Homology, Amino Acid Untranslated Regions - genetics |
| title | Cloning and characterization of the L15 ribosomal protein gene homologue from the crayfish Orconectes limosus |
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