Cloning and characterization of the L15 ribosomal protein gene homologue from the crayfish Orconectes limosus

A cDNA homologue of the large unit rat ribosomal protein L15 was cloned from an epidermal cDNA library of the crayfish Orconectes limosus, being the first crustacean ribosomal protein gene cloned to date. It contains 204 amino acids, deduced from the nucleotide sequence, and reveals 70-76% sequence...

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Veröffentlicht in:	Biochemical and biophysical research communications 1999-03, Vol.256 (2), p.313-316
Hauptverfasser:	Tom, M, Waterman, M R, Böcking, D, Dauphin-Villemant, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acids - chemistry Amino Acids - genetics Animals Astacoidea - genetics Base Sequence Blotting, Northern Cloning, Molecular Epidermis Freshwater Gene Expression Gene Library Humans Molecular Sequence Data Open Reading Frames - genetics Orconectes limosus Ribosomal Proteins - chemistry Ribosomal Proteins - genetics Sequence Alignment Sequence Homology, Amino Acid Untranslated Regions - genetics
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Beschreibung
Zusammenfassung:	A cDNA homologue of the large unit rat ribosomal protein L15 was cloned from an epidermal cDNA library of the crayfish Orconectes limosus, being the first crustacean ribosomal protein gene cloned to date. It contains 204 amino acids, deduced from the nucleotide sequence, and reveals 70-76% sequence identity with other arthropod and vertebrate ribosomal L15s. A single, abundant 0.7-kb mRNA transcript was constitutively expressed and revealed similar expression levels, among various adult crayfish tissues.
ISSN:	0006-291X
DOI:	10.1006/bbrc.1999.0322