Focal Sites of DNA Repair Synthesis in Human Chromosomes

Nucleotide excision repair (NER) is the principle pathway by which the human cells eliminate UV-induced lesions from their genomic DNA. The process can be visualized through the labelling of the nucleotides that are incoporated into repair patches, following the excision of the damaged stretch of DN...

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Veröffentlicht in:	Biochemical and biophysical research communications 1999-04, Vol.257 (2), p.378-383
Hauptverfasser:	Svetlova, Maria P., Solovjeva, Liudmila V., Pleskach, Nadezhda A., Tomilin, Nikolai V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biotin - analogs & derivatives Cell Line Cell Nucleus - genetics Cell Nucleus - radiation effects Chromosomes - genetics Chromosomes - radiation effects DNA - biosynthesis DNA - genetics DNA Damage - genetics DNA Damage - radiation effects DNA Repair - genetics DNA Repair - radiation effects DNA repair synthesis Fibroblasts - metabolism Fibroblasts - radiation effects Fluorescent Antibody Technique Fluorescent Dyes focal sites human fibroblasts Humans Idoxuridine - metabolism immunofluorescence Interphase Metaphase Skin Software Time Factors Tyramine - analogs & derivatives ultraviolet light Ultraviolet Rays
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Beschreibung
Zusammenfassung:	Nucleotide excision repair (NER) is the principle pathway by which the human cells eliminate UV-induced lesions from their genomic DNA. The process can be visualized through the labelling of the nucleotides that are incoporated into repair patches, following the excision of the damaged stretch of DNA. In this study we have visualized sites of DNA repair synthesis (DRS) in human interphase and metaphase chromosomes after very short times (2.5–30 min) of postirradiation labellingin vivowith 5-iododeoxyuridine. A limited number (
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1999.0485