Gender Aspects of Liver Slice Incubations with N, N, N‐Triethylene‐thiophosphamide (Thio‐TEPA) in Rats and Mice

This study was conducted to investigate if biotransformation of N, N, N‐triethylene‐thiophosphoramide (thio‐TEPA) by liver slice incubations reflects the established gender pattern for rat and mouse. Liver slices from rat and mice of both genders were incubated with start concentrations of thio‐TEPA of 5.2, 26, 52 and 104 μM for up to 240 min. Male rat liver slices eliminated thio‐TEPA faster and formed more TEPA than female liver slices at any concentration. No gender difference was found for the elimination of thio‐TEPA in mice, however, the female liver slices formed less TEPA than the male ones. Apparently female rat liver slices formed less TEPA than female mice liver slices. It is concluded that the liver slice incubation system in a robust manner reflects gender differences in rat drug biotransformation with special reference to thio‐TEPA. It is also confirmed that these aspects of gender are less pronounced in the examined mouse species than in rats.