In Situ Detection of Escherichia coli Cells Containing ColE1-related Plasmids by Hybridization to Regulatory RNA II

In Situ Detection of Escherichia coli Cells Containing ColE1-related Plasmids by Hybridization to Regulatory RNA II

A method is described for the in situ detection of individual whole fixed cells of Escherichia coli containing ColE1-related plasmids. It makes use of fluorescence in situ hybridization (FISH) and the regulatory RNA II as a target molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various...

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Veröffentlicht in:Systematic and applied microbiology 1999-02, Vol.22 (1), p.1-8
Hauptverfasser: Juretschko, Stefan, Schönhuber, Wilhelm, Kulakauskas, Saulius, Ehrlich, Dusko S., Schleifer, Karl-Heinz, Amann, Rudolf
Format: Artikel
Sprache:eng
Schlagworte:
ColE1-related plasmids
Escherichia coli
Escherichia coli - genetics
Escherichia coli - isolation & purification
fluorescence in situ hybridization
in situ detection
In Situ Hybridization, Fluorescence
Oligonucleotide Probes
Plasmids
RNA - genetics
RNA I
RNA II
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Beschreibung
Zusammenfassung:A method is described for the in situ detection of individual whole fixed cells of Escherichia coli containing ColE1-related plasmids. It makes use of fluorescence in situ hybridization (FISH) and the regulatory RNA II as a target molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various methods for signal amplification were compared. Probes targeting the regulatory RNA I did not result in the in situ detection of plasmid-bearing cells.
ISSN:0723-2020
1618-0984
DOI:10.1016/S0723-2020(99)80022-8