A plasmid-encoded two-component regulatory system involved in copper-inducible transcription in Lactococcus lactis
Two regulatory genes ( lcoR and lcoS) were identified from a plasmid-borne lactococcal copper resistance determinant and characterized by transcriptional fusion to the promoterless chloramphenicol acetyltransferase gene ( cat). RT–PCR analysis indicates that lcoR and lcoS are organized within an ope...
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Veröffentlicht in: | Gene 1999-03, Vol.229 (1), p.229-235 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Two regulatory genes (
lcoR and
lcoS) were identified from a plasmid-borne lactococcal copper resistance determinant and characterized by transcriptional fusion to the promoterless chloramphenicol acetyltransferase gene (
cat). RT–PCR analysis indicates that
lcoR and
lcoS are organized within an operon, controlling the transcription of
cat in a copper-inducible manner. The amino acid sequences deduced from
lcoR and
lcoS show homology to the response and sensor proteins of known two-component regulatory systems. Deletion within either
lcoS or both genes inactivated the copper-dependent activity, suggesting the presence of no trans-acting
lcoR and
lcoS homologs in the lactococcal host chromosome. The transcription start site involved in copper induction was mapped by primer extension. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/S0378-1119(98)00395-3 |