Regulation of capsular polysialic acid biosynthesis by temperature in Pasteurella haemolytica A2

The capsular polysaccharide of Pasteurella haemolytica A2 consists of a linear polymer of N-acetylneuraminic acid (Neu5Ac) with α(2–8) linkages. The production of this polymer is strictly regulated by the growth temperature and above 40°C no production is detected. Analysis of the enzymatic activiti...

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Veröffentlicht in:FEBS letters 1999-02, Vol.445 (2), p.325-328
Hauptverfasser: Barrallo, Sofia, Reglero, Angel, Revilla-Nuin, Beatriz, Martı́nez-Blanco, Honorina, Rodrı́guez-Aparicio, Leandro B, Ferrero, Miguel A
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Sprache:eng
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Zusammenfassung:The capsular polysaccharide of Pasteurella haemolytica A2 consists of a linear polymer of N-acetylneuraminic acid (Neu5Ac) with α(2–8) linkages. The production of this polymer is strictly regulated by the growth temperature and above 40°C no production is detected. Analysis of the enzymatic activities directly involved in its biosynthesis reveals that Neu5Ac lyase, CMP-Neu5Ac synthetase and polysialyltransferase are involved in this regulation. Very low activities were found in P. haemolytica grown at 43°C (at least 25 times lower than those observed when the growth temperature was 37°C). The synthesis of these enzymes increased rapidly when bacteria grown at 43°C were transferred to 37°C and decreased dramatically when cells grown at 37°C were transferred to 43°C. These findings indicate that the cellular growth temperature regulates the synthesis of these enzymes and hence the concentration of the intermediates necessary for capsular polysaccharide genesis in P. haemolytica A2.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(99)00163-5