Proof that the actVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin
Pyran ring formation in the biosynthesis of actinorhodin in Streptomyces coelicolor A3(2) was studied using the act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the actVI genes. The strain, CH999/pIJ5660 (pRM5 + actVI-ORF1), produced a chiral intermediate,...
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| Veröffentlicht in: | Bioorganic & medicinal chemistry letters 1999-02, Vol.9 (3), p.395-400 |
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| container_title | Bioorganic & medicinal chemistry letters |
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| creator | Ichinose, Koji Surti, Chandres Taguchi, Takaaki Malpartida, Francisco Booker-Milburm, Kevin I. Stephenson, G.Richard Ebizuka, Yutaka Hopwood, David A. |
| description | Pyran ring formation in the biosynthesis of actinorhodin in
Streptomyces coelicolor A3(2) was studied using the
act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the
actVI genes. The strain, CH999/pIJ5660 (pRM5 +
actVI-ORF1), produced a chiral intermediate, (
S)-DNPA, suggesting that the
actVI-ORF1 product is a reductase determining the C-3 stereochemical centre.
Pyran ring formation in the biosynthesis of actinorhodin in
Streptomyces coelicolor A3(2) was studied using the
act cluster deficient strain, CH999, carrying a series of pRM5-based plasmids harbouring combinations of the
actVI genes. The strain, CH999/pU5660 (pRM5 +
actVI-ORF1), produced a chiral biosynthetic intermediate, (
S)-DNPA, suggesting that the
actVI-ORF1 product is a reductase detemining the C-3 stereochemical centre. |
| doi_str_mv | 10.1016/S0960-894X(99)00011-6 |
| format | Article |
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Streptomyces coelicolor A3(2) was studied using the
act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the
actVI genes. The strain, CH999/pIJ5660 (pRM5 +
actVI-ORF1), produced a chiral intermediate, (
S)-DNPA, suggesting that the
actVI-ORF1 product is a reductase determining the C-3 stereochemical centre.
Pyran ring formation in the biosynthesis of actinorhodin in
Streptomyces coelicolor A3(2) was studied using the
act cluster deficient strain, CH999, carrying a series of pRM5-based plasmids harbouring combinations of the
actVI genes. The strain, CH999/pU5660 (pRM5 +
actVI-ORF1), produced a chiral biosynthetic intermediate, (
S)-DNPA, suggesting that the
actVI-ORF1 product is a reductase detemining the C-3 stereochemical centre.</description><identifier>ISSN: 0960-894X</identifier><identifier>EISSN: 1464-3405</identifier><identifier>DOI: 10.1016/S0960-894X(99)00011-6</identifier><identifier>PMID: 10091691</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Anthraquinones - chemistry ; Anthraquinones - metabolism ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Miscellaneous ; Mission oriented research ; Multigene Family ; Open Reading Frames ; Pyrans - chemistry ; Restriction Mapping ; Streptomyces - genetics</subject><ispartof>Bioorganic & medicinal chemistry letters, 1999-02, Vol.9 (3), p.395-400</ispartof><rights>1999</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-ddfc440665efd0bdcfc32ab7ea15902dabecb323eb7dbfc9cf55e514500b002b3</citedby><cites>FETCH-LOGICAL-c390t-ddfc440665efd0bdcfc32ab7ea15902dabecb323eb7dbfc9cf55e514500b002b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0960-894X(99)00011-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1717273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10091691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ichinose, Koji</creatorcontrib><creatorcontrib>Surti, Chandres</creatorcontrib><creatorcontrib>Taguchi, Takaaki</creatorcontrib><creatorcontrib>Malpartida, Francisco</creatorcontrib><creatorcontrib>Booker-Milburm, Kevin I.</creatorcontrib><creatorcontrib>Stephenson, G.Richard</creatorcontrib><creatorcontrib>Ebizuka, Yutaka</creatorcontrib><creatorcontrib>Hopwood, David A.</creatorcontrib><title>Proof that the actVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin</title><title>Bioorganic & medicinal chemistry letters</title><addtitle>Bioorg Med Chem Lett</addtitle><description>Pyran ring formation in the biosynthesis of actinorhodin in
Streptomyces coelicolor A3(2) was studied using the
act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the
actVI genes. The strain, CH999/pIJ5660 (pRM5 +
actVI-ORF1), produced a chiral intermediate, (
S)-DNPA, suggesting that the
actVI-ORF1 product is a reductase determining the C-3 stereochemical centre.
Pyran ring formation in the biosynthesis of actinorhodin in
Streptomyces coelicolor A3(2) was studied using the
act cluster deficient strain, CH999, carrying a series of pRM5-based plasmids harbouring combinations of the
actVI genes. The strain, CH999/pU5660 (pRM5 +
actVI-ORF1), produced a chiral biosynthetic intermediate, (
S)-DNPA, suggesting that the
actVI-ORF1 product is a reductase detemining the C-3 stereochemical centre.</description><subject>Anthraquinones - chemistry</subject><subject>Anthraquinones - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Miscellaneous</subject><subject>Mission oriented research</subject><subject>Multigene Family</subject><subject>Open Reading Frames</subject><subject>Pyrans - chemistry</subject><subject>Restriction Mapping</subject><subject>Streptomyces - genetics</subject><issn>0960-894X</issn><issn>1464-3405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2KFDEUhYMoTjv6CEoWIjOL0puqVJVZyTD4MzCgMCruQn5ueiJVSU2Sbuhn8WVNTzfqzk1yF98953IOIc8ZvGbAhjc3IAZo3gr-40yIcwBgrBkekBXjA286Dv1DsvqDnJAnOf-sDAfOH5MTBiDYINiK_PqSYnS03KpSH6TKlO9XdI0Bizc04drHQCtwUxIuJc47g5maiJM3cYqJXnRn7Tn1mfqwjdMWbR1oLpgw5gWNd1Vl2SUVaPJhTV1Msyp7zYrt_bSPeRfqlKtG9an-PsR0G60PT8kjp6aMz47_Kfn24f3Xy0_N9eePV5cX143pBJTGWmc4h2Ho0VnQ1jjTtUqPqFgvoLVKo9Fd26EerXZGGNf32DPeA2iAVnen5NVBd0nxboO5yNlng9OkAsZNloMYOKtKFewPoEkx54ROLsnPKu0kA7lvRd63IveRSyHkfStyqHsvjgYbPaP9Z-tQQwVeHgGVjZpczcv4_Jcb2diOXcXeHTCsaWw9JpmNx2DQ-oSmSBv9fy75DeX5rd4</recordid><startdate>19990208</startdate><enddate>19990208</enddate><creator>Ichinose, Koji</creator><creator>Surti, Chandres</creator><creator>Taguchi, Takaaki</creator><creator>Malpartida, Francisco</creator><creator>Booker-Milburm, Kevin I.</creator><creator>Stephenson, G.Richard</creator><creator>Ebizuka, Yutaka</creator><creator>Hopwood, David A.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990208</creationdate><title>Proof that the actVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin</title><author>Ichinose, Koji ; Surti, Chandres ; Taguchi, Takaaki ; Malpartida, Francisco ; Booker-Milburm, Kevin I. ; Stephenson, G.Richard ; Ebizuka, Yutaka ; Hopwood, David A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-ddfc440665efd0bdcfc32ab7ea15902dabecb323eb7dbfc9cf55e514500b002b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Anthraquinones - chemistry</topic><topic>Anthraquinones - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Miscellaneous</topic><topic>Mission oriented research</topic><topic>Multigene Family</topic><topic>Open Reading Frames</topic><topic>Pyrans - chemistry</topic><topic>Restriction Mapping</topic><topic>Streptomyces - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ichinose, Koji</creatorcontrib><creatorcontrib>Surti, Chandres</creatorcontrib><creatorcontrib>Taguchi, Takaaki</creatorcontrib><creatorcontrib>Malpartida, Francisco</creatorcontrib><creatorcontrib>Booker-Milburm, Kevin I.</creatorcontrib><creatorcontrib>Stephenson, G.Richard</creatorcontrib><creatorcontrib>Ebizuka, Yutaka</creatorcontrib><creatorcontrib>Hopwood, David A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioorganic & medicinal chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ichinose, Koji</au><au>Surti, Chandres</au><au>Taguchi, Takaaki</au><au>Malpartida, Francisco</au><au>Booker-Milburm, Kevin I.</au><au>Stephenson, G.Richard</au><au>Ebizuka, Yutaka</au><au>Hopwood, David A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proof that the actVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin</atitle><jtitle>Bioorganic & medicinal chemistry letters</jtitle><addtitle>Bioorg Med Chem Lett</addtitle><date>1999-02-08</date><risdate>1999</risdate><volume>9</volume><issue>3</issue><spage>395</spage><epage>400</epage><pages>395-400</pages><issn>0960-894X</issn><eissn>1464-3405</eissn><abstract>Pyran ring formation in the biosynthesis of actinorhodin in
Streptomyces coelicolor A3(2) was studied using the
act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the
actVI genes. The strain, CH999/pIJ5660 (pRM5 +
actVI-ORF1), produced a chiral intermediate, (
S)-DNPA, suggesting that the
actVI-ORF1 product is a reductase determining the C-3 stereochemical centre.
Pyran ring formation in the biosynthesis of actinorhodin in
Streptomyces coelicolor A3(2) was studied using the
act cluster deficient strain, CH999, carrying a series of pRM5-based plasmids harbouring combinations of the
actVI genes. The strain, CH999/pU5660 (pRM5 +
actVI-ORF1), produced a chiral biosynthetic intermediate, (
S)-DNPA, suggesting that the
actVI-ORF1 product is a reductase detemining the C-3 stereochemical centre.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10091691</pmid><doi>10.1016/S0960-894X(99)00011-6</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-894X |
| ispartof | Bioorganic & medicinal chemistry letters, 1999-02, Vol.9 (3), p.395-400 |
| issn | 0960-894X 1464-3405 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Anthraquinones - chemistry Anthraquinones - metabolism Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Fundamental and applied biological sciences. Psychology Genes, Bacterial Miscellaneous Mission oriented research Multigene Family Open Reading Frames Pyrans - chemistry Restriction Mapping Streptomyces - genetics |
| title | Proof that the actVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin |
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