Proof that the actVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin

Proof that the actVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin

Pyran ring formation in the biosynthesis of actinorhodin in Streptomyces coelicolor A3(2) was studied using the act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the actVI genes. The strain, CH999/pIJ5660 (pRM5 + actVI-ORF1), produced a chiral intermediate,...

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Veröffentlicht in:Bioorganic & medicinal chemistry letters 1999-02, Vol.9 (3), p.395-400
Hauptverfasser: Ichinose, Koji, Surti, Chandres, Taguchi, Takaaki, Malpartida, Francisco, Booker-Milburm, Kevin I., Stephenson, G.Richard, Ebizuka, Yutaka, Hopwood, David A.
Format: Artikel
Sprache:eng
Schlagworte:
Anthraquinones - chemistry
Anthraquinones - metabolism
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Miscellaneous
Mission oriented research
Multigene Family
Open Reading Frames
Pyrans - chemistry
Restriction Mapping
Streptomyces - genetics
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Zusammenfassung:Pyran ring formation in the biosynthesis of actinorhodin in Streptomyces coelicolor A3(2) was studied using the act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the actVI genes. The strain, CH999/pIJ5660 (pRM5 + actVI-ORF1), produced a chiral intermediate, ( S)-DNPA, suggesting that the actVI-ORF1 product is a reductase determining the C-3 stereochemical centre. Pyran ring formation in the biosynthesis of actinorhodin in Streptomyces coelicolor A3(2) was studied using the act cluster deficient strain, CH999, carrying a series of pRM5-based plasmids harbouring combinations of the actVI genes. The strain, CH999/pU5660 (pRM5 + actVI-ORF1), produced a chiral biosynthetic intermediate, ( S)-DNPA, suggesting that the actVI-ORF1 product is a reductase detemining the C-3 stereochemical centre.
ISSN:0960-894X
1464-3405
DOI:10.1016/S0960-894X(99)00011-6