Rheinheimera soli sp. nov., a gammaproteobacterium isolated from soil in Korea

1 Division of Applied Life Science (BK21 Program), EB-NCRC, Gyeongsang National University, Jinju 660-701, Republic of Korea 2 Department of Animal Science & Technology, Sunchon National University, Sunchon 540-742, Republic of Korea 3 Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea Correspondence Che Ok Jeon cojeon{at}cau.ac.kr A Gram-negative, non-spore-forming bacterium, designated strain BD-d46 T , was isolated from a playground soil sample in Jinju, South Korea. Cells were straight or curved rods and showed catalase- and oxidase-positive reactions. Growth of strain BD-d46 T was observed between 15 and 35 °C (optimum 25–30 °C) and between pH 6.5 and 8.0 (optimum pH 7.0–7.5). The predominant fatty acids were summed feature 3 (C 16 : 1 7 c and/or iso-C 15 : 0 2-OH), C 12 : 0 3-OH and C 16 : 0 . Strain BD-d46 T contained phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. Isoprenoid quinones were Q-8 (75 %) and MK-7 (25 %). The G+C content of the genomic DNA was 49.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BD-d46 T formed a distinct lineage with Rheinheimera chironomi K19414 T within the genus Rheinheimera . Levels of 16S rRNA gene sequence similarity between strain BD-d46 T and the type strains of recognized Rheinheimera species ranged from 94.4 to 96.9 %. On the basis of chemotaxonomic data and molecular properties, strain BD-d46 T is considered to represent a novel species of the genus Rheinheimera , for which the name Rheinheimera soli sp. nov. is proposed. The type strain is BD-d46 T (=KCTC 22077 T =DSM 19413 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain BD-d46 T is EF575565 . A transmission electron micrograph of a negatively stained cell and a table detailing the cellular fatty acid composition of strain BD-d46 T and TLC of polar lipids of strain BD-d46 T and R. chironomi K19414 T are available as supplementary material with the online version of this paper.