B Cell Response After MMTV Infection: Extrafollicular Plasmablasts Represent the Main Infected Population and Can Transmit Viral Infection

The immune response to mouse mammary tumor virus (MMTV) relies on the presentation of an MMTV-encoded superantigen by infected B cells to superantigen-specific T cells. The initial extrafollicular B cell differentiation involved the generation of B cells expressing low levels of B220. These B220low...

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Veröffentlicht in:The Journal of immunology (1950) 1999-03, Vol.162 (5), p.2538-2545
Hauptverfasser: Ardavin, Carlos, Martin, Pilar, Ferrero, Isabel, Azcoitia, Inigo, Anjuere, Fabienne, Diggelmann, Heidi, Luthi, Frederic, Luther, Sanjiv, Acha-Orbea, Hans
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Sprache:eng
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Zusammenfassung:The immune response to mouse mammary tumor virus (MMTV) relies on the presentation of an MMTV-encoded superantigen by infected B cells to superantigen-specific T cells. The initial extrafollicular B cell differentiation involved the generation of B cells expressing low levels of B220. These B220low B cells corresponded to plasmablasts that expressed high levels of CD43 and syndecan-1 and were CD62 ligand- and IgD-. Viral DNA was detected nearly exclusively in these B220low B cells by PCR, and retroviral type-A particles were observed in their cytoplasm by electron microscopy. An MMTV transmission to the offspring was also achieved after transfer of B220low CD62 ligand- CD43+ plasmablasts into noninfected females. These data suggest that B220low plasmablasts, representing the bulk of infected B cells, are capable of sustaining viral replication and may be involved in the transmission of MMTV.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.162.5.2538