Differential expression of tumor necrosis factor receptor subtypes on leukocytes in systemic inflammatory response syndrome
OBJECTIVETo determine the expression of tumor necrosis factor (TNF) receptor in patients with systemic inflammatory response syndrome (SIRS). DESIGNProspective study. SETTINGIntensive care unit and central laboratory. PATIENTSBlood specimens from 18 healthy volunteers (controls) and 16 patients with...
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| Veröffentlicht in: | Critical care medicine 1999-02, Vol.27 (2), p.319-324 |
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| creator | Hubl, Wolfgang Wolfbauer, Georg Streicher, Johannes Andert, Sylvia Stanek, Gunther Fitzal, Sylvia Bayer, Peter Michael |
| description | OBJECTIVETo determine the expression of tumor necrosis factor (TNF) receptor in patients with systemic inflammatory response syndrome (SIRS).
DESIGNProspective study.
SETTINGIntensive care unit and central laboratory.
PATIENTSBlood specimens from 18 healthy volunteers (controls) and 16 patients with SIRS.
INTERVENTIONSNone.
MEASUREMENTS AND MAIN RESULTSUsing monoclonal antibodies, fluorescence labeling, and high sensitivity flow cytometry, we measured the expression of membrane TNF receptor subtypes TNF-R55 and TNF-R75 on peripheral blood leukocytes. Receptor expression is expressed as mean fluorescence intensity +/- SD (unitsdetection channel number). In controls, TNF-R55 was only weakly expressed (monocytes2.5 +/- 1.8; neutrophils0.7 +/- 0.8), whereas expression of TNF-R75 was higher (monocytes28.6 +/- 9.0; neutrophils4.8 +/- 1.0) and was also found on lymphocytes (on CD8 lymphocytes5.7 +/- 1.8; CD165.5 +/- 1.2; CD49.7 +/- 3.7). In SIRS, we observed increased expression of TNF-R55 on monocytes (6.9 +/- 3.4, p < .001) and neutrophils (2.2 +/- 1.9, p < .01), as well as decreased expression of TNF-R75 on monocytes (17.3 +/- 13.2; p < .001). The extent of TNF-R55 up-regulation did not correlate with that of TNF-R75 down-regulation. TNF-R55 on monocytes and neutrophils strongly correlated with body temperature but not with survival, whereas monocyte TNF-R75 was considerably lower in nonsurvivors, albeit not significantly (12.3 +/- 7.1 vs. 23.9 +/- 16.7; p = .07).
CONCLUSIONSThese data indicate that leukocyte TNF-R55 and TNF-R75 react differentially and probably serve different functions in SIRS, which prompts the investigation of receptor subtype-specific therapeutic approaches. (Crit Care Med 1999; 27:319-324) |
| doi_str_mv | 10.1097/00003246-199902000-00038 |
| format | Article |
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DESIGNProspective study.
SETTINGIntensive care unit and central laboratory.
PATIENTSBlood specimens from 18 healthy volunteers (controls) and 16 patients with SIRS.
INTERVENTIONSNone.
MEASUREMENTS AND MAIN RESULTSUsing monoclonal antibodies, fluorescence labeling, and high sensitivity flow cytometry, we measured the expression of membrane TNF receptor subtypes TNF-R55 and TNF-R75 on peripheral blood leukocytes. Receptor expression is expressed as mean fluorescence intensity +/- SD (unitsdetection channel number). In controls, TNF-R55 was only weakly expressed (monocytes2.5 +/- 1.8; neutrophils0.7 +/- 0.8), whereas expression of TNF-R75 was higher (monocytes28.6 +/- 9.0; neutrophils4.8 +/- 1.0) and was also found on lymphocytes (on CD8 lymphocytes5.7 +/- 1.8; CD165.5 +/- 1.2; CD49.7 +/- 3.7). In SIRS, we observed increased expression of TNF-R55 on monocytes (6.9 +/- 3.4, p < .001) and neutrophils (2.2 +/- 1.9, p < .01), as well as decreased expression of TNF-R75 on monocytes (17.3 +/- 13.2; p < .001). The extent of TNF-R55 up-regulation did not correlate with that of TNF-R75 down-regulation. TNF-R55 on monocytes and neutrophils strongly correlated with body temperature but not with survival, whereas monocyte TNF-R75 was considerably lower in nonsurvivors, albeit not significantly (12.3 +/- 7.1 vs. 23.9 +/- 16.7; p = .07).
CONCLUSIONSThese data indicate that leukocyte TNF-R55 and TNF-R75 react differentially and probably serve different functions in SIRS, which prompts the investigation of receptor subtype-specific therapeutic approaches. (Crit Care Med 1999; 27:319-324)</description><identifier>ISSN: 0090-3493</identifier><identifier>EISSN: 1530-0293</identifier><identifier>DOI: 10.1097/00003246-199902000-00038</identifier><identifier>PMID: 10075056</identifier><identifier>CODEN: CCMDC7</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins, Inc</publisher><subject>Adult ; Aged ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Antibodies - blood ; Biological and medical sciences ; Emergency and intensive care: infection, septic shock ; Female ; Flow Cytometry - methods ; Flow Cytometry - statistics & numerical data ; Fluorescent Antibody Technique - statistics & numerical data ; Humans ; Intensive care medicine ; Leukocytes - classification ; Leukocytes - physiology ; Linear Models ; Male ; Medical sciences ; Middle Aged ; Molecular Weight ; Prospective Studies ; Receptors, Tumor Necrosis Factor - blood ; Receptors, Tumor Necrosis Factor - classification ; Receptors, Tumor Necrosis Factor - immunology ; Reference Values ; Statistics, Nonparametric ; Systemic Inflammatory Response Syndrome - blood</subject><ispartof>Critical care medicine, 1999-02, Vol.27 (2), p.319-324</ispartof><rights>1999 Lippincott Williams & Wilkins, Inc.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3858-53b6161755d5f30e4dd73c36d3f812f6f733983821c0736b61045d4f0fd15d333</citedby><cites>FETCH-LOGICAL-c3858-53b6161755d5f30e4dd73c36d3f812f6f733983821c0736b61045d4f0fd15d333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1704380$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10075056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hubl, Wolfgang</creatorcontrib><creatorcontrib>Wolfbauer, Georg</creatorcontrib><creatorcontrib>Streicher, Johannes</creatorcontrib><creatorcontrib>Andert, Sylvia</creatorcontrib><creatorcontrib>Stanek, Gunther</creatorcontrib><creatorcontrib>Fitzal, Sylvia</creatorcontrib><creatorcontrib>Bayer, Peter Michael</creatorcontrib><title>Differential expression of tumor necrosis factor receptor subtypes on leukocytes in systemic inflammatory response syndrome</title><title>Critical care medicine</title><addtitle>Crit Care Med</addtitle><description>OBJECTIVETo determine the expression of tumor necrosis factor (TNF) receptor in patients with systemic inflammatory response syndrome (SIRS).
DESIGNProspective study.
SETTINGIntensive care unit and central laboratory.
PATIENTSBlood specimens from 18 healthy volunteers (controls) and 16 patients with SIRS.
INTERVENTIONSNone.
MEASUREMENTS AND MAIN RESULTSUsing monoclonal antibodies, fluorescence labeling, and high sensitivity flow cytometry, we measured the expression of membrane TNF receptor subtypes TNF-R55 and TNF-R75 on peripheral blood leukocytes. Receptor expression is expressed as mean fluorescence intensity +/- SD (unitsdetection channel number). In controls, TNF-R55 was only weakly expressed (monocytes2.5 +/- 1.8; neutrophils0.7 +/- 0.8), whereas expression of TNF-R75 was higher (monocytes28.6 +/- 9.0; neutrophils4.8 +/- 1.0) and was also found on lymphocytes (on CD8 lymphocytes5.7 +/- 1.8; CD165.5 +/- 1.2; CD49.7 +/- 3.7). In SIRS, we observed increased expression of TNF-R55 on monocytes (6.9 +/- 3.4, p < .001) and neutrophils (2.2 +/- 1.9, p < .01), as well as decreased expression of TNF-R75 on monocytes (17.3 +/- 13.2; p < .001). The extent of TNF-R55 up-regulation did not correlate with that of TNF-R75 down-regulation. TNF-R55 on monocytes and neutrophils strongly correlated with body temperature but not with survival, whereas monocyte TNF-R75 was considerably lower in nonsurvivors, albeit not significantly (12.3 +/- 7.1 vs. 23.9 +/- 16.7; p = .07).
CONCLUSIONSThese data indicate that leukocyte TNF-R55 and TNF-R75 react differentially and probably serve different functions in SIRS, which prompts the investigation of receptor subtype-specific therapeutic approaches. (Crit Care Med 1999; 27:319-324)</description><subject>Adult</subject><subject>Aged</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Antibodies - blood</subject><subject>Biological and medical sciences</subject><subject>Emergency and intensive care: infection, septic shock</subject><subject>Female</subject><subject>Flow Cytometry - methods</subject><subject>Flow Cytometry - statistics & numerical data</subject><subject>Fluorescent Antibody Technique - statistics & numerical data</subject><subject>Humans</subject><subject>Intensive care medicine</subject><subject>Leukocytes - classification</subject><subject>Leukocytes - physiology</subject><subject>Linear Models</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular Weight</subject><subject>Prospective Studies</subject><subject>Receptors, Tumor Necrosis Factor - blood</subject><subject>Receptors, Tumor Necrosis Factor - classification</subject><subject>Receptors, Tumor Necrosis Factor - immunology</subject><subject>Reference Values</subject><subject>Statistics, Nonparametric</subject><subject>Systemic Inflammatory Response Syndrome - blood</subject><issn>0090-3493</issn><issn>1530-0293</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtv1TAQhS1ERS8tfwFlgdgFxpnYSZaolIdUiU27tnydsRrqxMGTqET8eXy5l8cGbzxn_B1bcyxEIeGNhK55C3lhVetSdl0HVVblodM-ETupMIuqw6diB9BBiXWH5-I581cAWasGn4lzCdAoUHonfrwfvKdE0zLYUND3ORHzEKci-mJZx5iKiVyKPHDhrVuyTuRoPhS87pdtJi4yHWh9iG5bshqmgjdeaBxcrn2w42gzvmUjz3FiysdTn-JIl-LM28D04rRfiLsP17dXn8qbLx8_X727KR22qi0V7rXUslGqVx6B6r5v0KHu0bey8to3iF2LbSUdNKgzDLXqaw--l6pHxAvx-njvnOK3lXgx48COQrATxZWN7rTEptYZbI_gYWJO5M2chtGmzUgwh-DN7-DNn-DNr-Cz9eXpjXU_Uv-P8Zh0Bl6dAMvOBp_s5Ab-yzVQYwsZq4_YYwwLJX4I6yMlc082LPfmf_-OPwEoNpzW</recordid><startdate>199902</startdate><enddate>199902</enddate><creator>Hubl, Wolfgang</creator><creator>Wolfbauer, Georg</creator><creator>Streicher, Johannes</creator><creator>Andert, Sylvia</creator><creator>Stanek, Gunther</creator><creator>Fitzal, Sylvia</creator><creator>Bayer, Peter Michael</creator><general>Lippincott Williams & Wilkins, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199902</creationdate><title>Differential expression of tumor necrosis factor receptor subtypes on leukocytes in systemic inflammatory response syndrome</title><author>Hubl, Wolfgang ; Wolfbauer, Georg ; Streicher, Johannes ; Andert, Sylvia ; Stanek, Gunther ; Fitzal, Sylvia ; Bayer, Peter Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3858-53b6161755d5f30e4dd73c36d3f812f6f733983821c0736b61045d4f0fd15d333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Antibodies - blood</topic><topic>Biological and medical sciences</topic><topic>Emergency and intensive care: infection, septic shock</topic><topic>Female</topic><topic>Flow Cytometry - methods</topic><topic>Flow Cytometry - statistics & numerical data</topic><topic>Fluorescent Antibody Technique - statistics & numerical data</topic><topic>Humans</topic><topic>Intensive care medicine</topic><topic>Leukocytes - classification</topic><topic>Leukocytes - physiology</topic><topic>Linear Models</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular Weight</topic><topic>Prospective Studies</topic><topic>Receptors, Tumor Necrosis Factor - blood</topic><topic>Receptors, Tumor Necrosis Factor - classification</topic><topic>Receptors, Tumor Necrosis Factor - immunology</topic><topic>Reference Values</topic><topic>Statistics, Nonparametric</topic><topic>Systemic Inflammatory Response Syndrome - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hubl, Wolfgang</creatorcontrib><creatorcontrib>Wolfbauer, Georg</creatorcontrib><creatorcontrib>Streicher, Johannes</creatorcontrib><creatorcontrib>Andert, Sylvia</creatorcontrib><creatorcontrib>Stanek, Gunther</creatorcontrib><creatorcontrib>Fitzal, Sylvia</creatorcontrib><creatorcontrib>Bayer, Peter Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Critical care medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hubl, Wolfgang</au><au>Wolfbauer, Georg</au><au>Streicher, Johannes</au><au>Andert, Sylvia</au><au>Stanek, Gunther</au><au>Fitzal, Sylvia</au><au>Bayer, Peter Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of tumor necrosis factor receptor subtypes on leukocytes in systemic inflammatory response syndrome</atitle><jtitle>Critical care medicine</jtitle><addtitle>Crit Care Med</addtitle><date>1999-02</date><risdate>1999</risdate><volume>27</volume><issue>2</issue><spage>319</spage><epage>324</epage><pages>319-324</pages><issn>0090-3493</issn><eissn>1530-0293</eissn><coden>CCMDC7</coden><abstract>OBJECTIVETo determine the expression of tumor necrosis factor (TNF) receptor in patients with systemic inflammatory response syndrome (SIRS).
DESIGNProspective study.
SETTINGIntensive care unit and central laboratory.
PATIENTSBlood specimens from 18 healthy volunteers (controls) and 16 patients with SIRS.
INTERVENTIONSNone.
MEASUREMENTS AND MAIN RESULTSUsing monoclonal antibodies, fluorescence labeling, and high sensitivity flow cytometry, we measured the expression of membrane TNF receptor subtypes TNF-R55 and TNF-R75 on peripheral blood leukocytes. Receptor expression is expressed as mean fluorescence intensity +/- SD (unitsdetection channel number). In controls, TNF-R55 was only weakly expressed (monocytes2.5 +/- 1.8; neutrophils0.7 +/- 0.8), whereas expression of TNF-R75 was higher (monocytes28.6 +/- 9.0; neutrophils4.8 +/- 1.0) and was also found on lymphocytes (on CD8 lymphocytes5.7 +/- 1.8; CD165.5 +/- 1.2; CD49.7 +/- 3.7). In SIRS, we observed increased expression of TNF-R55 on monocytes (6.9 +/- 3.4, p < .001) and neutrophils (2.2 +/- 1.9, p < .01), as well as decreased expression of TNF-R75 on monocytes (17.3 +/- 13.2; p < .001). The extent of TNF-R55 up-regulation did not correlate with that of TNF-R75 down-regulation. TNF-R55 on monocytes and neutrophils strongly correlated with body temperature but not with survival, whereas monocyte TNF-R75 was considerably lower in nonsurvivors, albeit not significantly (12.3 +/- 7.1 vs. 23.9 +/- 16.7; p = .07).
CONCLUSIONSThese data indicate that leukocyte TNF-R55 and TNF-R75 react differentially and probably serve different functions in SIRS, which prompts the investigation of receptor subtype-specific therapeutic approaches. (Crit Care Med 1999; 27:319-324)</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>10075056</pmid><doi>10.1097/00003246-199902000-00038</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Aged Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Antibodies - blood Biological and medical sciences Emergency and intensive care: infection, septic shock Female Flow Cytometry - methods Flow Cytometry - statistics & numerical data Fluorescent Antibody Technique - statistics & numerical data Humans Intensive care medicine Leukocytes - classification Leukocytes - physiology Linear Models Male Medical sciences Middle Aged Molecular Weight Prospective Studies Receptors, Tumor Necrosis Factor - blood Receptors, Tumor Necrosis Factor - classification Receptors, Tumor Necrosis Factor - immunology Reference Values Statistics, Nonparametric Systemic Inflammatory Response Syndrome - blood |
| title | Differential expression of tumor necrosis factor receptor subtypes on leukocytes in systemic inflammatory response syndrome |
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