Differential expression of tumor necrosis factor receptor subtypes on leukocytes in systemic inflammatory response syndrome
OBJECTIVETo determine the expression of tumor necrosis factor (TNF) receptor in patients with systemic inflammatory response syndrome (SIRS). DESIGNProspective study. SETTINGIntensive care unit and central laboratory. PATIENTSBlood specimens from 18 healthy volunteers (controls) and 16 patients with...
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Veröffentlicht in: | Critical care medicine 1999-02, Vol.27 (2), p.319-324 |
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Zusammenfassung: | OBJECTIVETo determine the expression of tumor necrosis factor (TNF) receptor in patients with systemic inflammatory response syndrome (SIRS).
DESIGNProspective study.
SETTINGIntensive care unit and central laboratory.
PATIENTSBlood specimens from 18 healthy volunteers (controls) and 16 patients with SIRS.
INTERVENTIONSNone.
MEASUREMENTS AND MAIN RESULTSUsing monoclonal antibodies, fluorescence labeling, and high sensitivity flow cytometry, we measured the expression of membrane TNF receptor subtypes TNF-R55 and TNF-R75 on peripheral blood leukocytes. Receptor expression is expressed as mean fluorescence intensity +/- SD (unitsdetection channel number). In controls, TNF-R55 was only weakly expressed (monocytes2.5 +/- 1.8; neutrophils0.7 +/- 0.8), whereas expression of TNF-R75 was higher (monocytes28.6 +/- 9.0; neutrophils4.8 +/- 1.0) and was also found on lymphocytes (on CD8 lymphocytes5.7 +/- 1.8; CD165.5 +/- 1.2; CD49.7 +/- 3.7). In SIRS, we observed increased expression of TNF-R55 on monocytes (6.9 +/- 3.4, p < .001) and neutrophils (2.2 +/- 1.9, p < .01), as well as decreased expression of TNF-R75 on monocytes (17.3 +/- 13.2; p < .001). The extent of TNF-R55 up-regulation did not correlate with that of TNF-R75 down-regulation. TNF-R55 on monocytes and neutrophils strongly correlated with body temperature but not with survival, whereas monocyte TNF-R75 was considerably lower in nonsurvivors, albeit not significantly (12.3 +/- 7.1 vs. 23.9 +/- 16.7; p = .07).
CONCLUSIONSThese data indicate that leukocyte TNF-R55 and TNF-R75 react differentially and probably serve different functions in SIRS, which prompts the investigation of receptor subtype-specific therapeutic approaches. (Crit Care Med 1999; 27:319-324) |
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ISSN: | 0090-3493 1530-0293 |
DOI: | 10.1097/00003246-199902000-00038 |