The influence of different hormone concentration and combination on callus induction and regeneration of Rauwolfia serpentina L. Benth

The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L(-1) BA and...

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Veröffentlicht in:	Pakistan journal of biological sciences 2008-06, Vol.11 (12), p.1638-1641
Hauptverfasser:	Salma, U, Rahman, M S M, Islam, S, Haque, N, Jubair, T A, Haque, A K M F, Mukti, I J
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaloids - chemistry Cell Communication - drug effects Cell Differentiation - drug effects Genotype Hydrogen-Ion Concentration Plant Growth Regulators - metabolism Plant Growth Regulators - pharmacology Plant Roots - metabolism Plant Shoots - metabolism Rauwolfia - drug effects Rauwolfia - physiology Regeneration - drug effects Temperature Time Factors Tissue Culture Techniques
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Zusammenfassung:	The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L(-1) BA and 2.0 mg L(-1) NAA. The callus differentiated into adventitious shoots when it was subcultured on MS medium supplemented with 2.0 mg L(-1) BA with 0.2 mg L(-1) NAA. Regenerated shoots were best rooted on half-strength MS medium with 1.0 mg L(-1) each of IBA and IAA.
ISSN:	1028-8880
DOI:	10.3923/pjbs.2008.1638.1641