Design and implementation of a protocol for the detection of Legionella in clinical and environmental samples

Abstract Our laboratory has developed a novel real-time polymerase chain reaction (PCR) assay for the detection of Legionella pneumophila and differentiation from other Legionella spp. in clinical and environmental samples. The 23S rRNA gene was used as a target to detect all Legionella spp., and th...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2008-10, Vol.62 (2), p.125-132
Hauptverfasser:	Nazarian, Elizabeth J, Bopp, Dianna J, Saylors, Amy, Limberger, Ronald J, Musser, Kimberlee A
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Bacteriology Biological and medical sciences Culture Media Environmental Microbiology Fundamental and applied biological sciences. Psychology Humans Infectious Disease Infectious diseases Internal Medicine Legionella Legionella - classification Legionella - genetics Legionella - isolation & purification Legionella pneumophila Legionella pneumophila - classification Legionella pneumophila - genetics Legionella pneumophila - isolation & purification Legionellosis - diagnosis Legionellosis - microbiology Legionnaires' Disease - diagnosis Legionnaires' Disease - microbiology Medical sciences Microbiology Miscellaneous Polymerase Chain Reaction - methods Protocol Real-time PCR RNA, Ribosomal, 23S - genetics Sensitivity and Specificity Sequence Analysis, DNA Time Factors
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Zusammenfassung:	Abstract Our laboratory has developed a novel real-time polymerase chain reaction (PCR) assay for the detection of Legionella pneumophila and differentiation from other Legionella spp. in clinical and environmental samples. The 23S rRNA gene was used as a target to detect all Legionella spp., and the mip gene was targeted for the specific detection of L. pneumophila in this multiplex Taqman® real-time PCR assay. The 23S rRNA gene is a novel target for Legionella testing; it detects all species and serogroups of Legionella without the contamination issues that accompany the use of the 16S rRNA gene as a target. This assay provides an analytical sensitivity of
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2008.05.004