Identification and Characterization of Nuclear Pore Subcomplexes in Mitotic Extract of Human Somatic Cells

In higher eukaryotic cells, it is generally thought that nuclear pore complex disassembles at the beginning of mitosis and reassembles at the end. Using a monoclonal antibody that recognizes nuclear pore antigens, we found that, at mitosis of mammalian somatic Hela cells, the nuclear pore complex di...

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Veröffentlicht in:	Biochemical and biophysical research communications 1999-01, Vol.254 (2), p.417-423
Hauptverfasser:	Matsuoka, Yosuke, Takagi, Masatoshi, Ban, Tadanobu, Miyazaki, Masanori, Yamamoto, Takahiro, Kondo, Yukihiro, Yoneda, Yoshihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies Antibodies, Monoclonal Cell Line Fluorescent Antibody Technique HeLa Cells Humans Membrane Proteins - analysis Membrane Proteins - chemistry Microscopy, Immunoelectron Mitosis Molecular Sequence Data Molecular Weight Nuclear Envelope - chemistry Nuclear Envelope - ultrastructure Nuclear Pore Complex Proteins Nuclear Proteins - analysis Nuclear Proteins - chemistry Peptide Fragments - chemistry Rats
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Zusammenfassung:	In higher eukaryotic cells, it is generally thought that nuclear pore complex disassembles at the beginning of mitosis and reassembles at the end. Using a monoclonal antibody that recognizes nuclear pore antigens, we found that, at mitosis of mammalian somatic Hela cells, the nuclear pore complex disassembles into at least three subcomplexes, termed subcomplexes A, B and C (molecular mass; 2 MDa
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1998.9953