Growth of Salinispora tropica strains CNB440, CNB476, and NPS21184 in nonsaline, low-sodium media
We recently described the development of a potassium-chloride-based salt formulation containing low sodium concentration (5.0 mM) to support the growth of Salinispora tropica strain NPS21184 and its production of salinosporamide A (NPI-0052). In order to determine whether the above low-sodium salt f...
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Veröffentlicht in: | Applied microbiology and biotechnology 2008-10, Vol.80 (5), p.873-880 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We recently described the development of a potassium-chloride-based salt formulation containing low sodium concentration (5.0 mM) to support the growth of
Salinispora tropica
strain NPS21184 and its production of salinosporamide A (NPI-0052). In order to determine whether the above low-sodium salt formulation can also support the growth of other
S
.
tropica
strains, we examined the growth of the type strain CNB440 and the parent strain CNB476, from which strain NPS21184 was derived as a single colony isolate. We demonstrated that good growth rate and yield of
S
.
tropica
strains CNB440 and CNB476, similar to
S
.
tropica
strain NPS21184 reported earlier, were detected in both agar and liquid media containing the potassium-chloride-based salt formulation with sodium concentration of 5.0 mM. Furthermore, we also detected good growth rate and yield of all three
S
.
tropica
strains on potassium-sulfate-based salt formulation agar medium containing both low-sodium (5.7 mM) and low-chloride (14 mM) content. This finding confirms the observation that the species of
S
.
tropica
does not have a seawater growth requirement but requirement for a specific combination of salts to provide a balance of salts and maintain a high enough ionic strength for growth. |
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ISSN: | 0175-7598 1432-0614 |
DOI: | 10.1007/s00253-008-1614-z |