Combination of immunohistochemistry and laser ablation ICP mass spectrometry for imaging of cancer biomarkers

Laser ablation (LA) ICP-MS has been developed as a new tool for imaging of cancer biomarkers in tissue sections. The distribution of two breast cancer-associated proteins, MUC-1 and HER2 was studied based on multiple line rastering of tissue sections and measurement of relevant Au/Ag tagged antibodi...

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Veröffentlicht in:	Proteomics (Weinheim) 2008-09, Vol.8 (18), p.3775-3784
Hauptverfasser:	Seuma, Jaume, Bunch, Josephine, Cox, Alan, McLeod, Cameron, Bell, Jane, Murray, Cliff
Format:	Artikel
Sprache:	eng
Schlagworte:	Biomarkers, Tumor - metabolism Breast - metabolism Breast - pathology Breast Neoplasms - metabolism Breast Neoplasms - pathology Cancer biomarkers Cellular imaging Elemental (Au/Ag) tagged antibodies Female Gastric Mucosa - metabolism Gastric Mucosa - pathology HER2 Humans Immunohistochemistry - methods Laser ablation ICP mass spectrometry Laser Therapy - methods Mucin-1 - metabolism Receptor, ErbB-2 - metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Beschreibung
Zusammenfassung:	Laser ablation (LA) ICP-MS has been developed as a new tool for imaging of cancer biomarkers in tissue sections. The distribution of two breast cancer-associated proteins, MUC-1 and HER2 was studied based on multiple line rastering of tissue sections and measurement of relevant Au/Ag tagged antibodies bound to the tissue. Comparisons with optical microscopy indicated extremely high sensitivity for the LA technique and sufficiently good resolution to permit fine scale feature mapping at the cellular level. Application to the quantitative assessment of HER2 expression in tissue microarrays was demonstrated.
ISSN:	1615-9853 1615-9861
DOI:	10.1002/pmic.200800167