Direct quantification of CSF α-synuclein by ELISA and first cross-sectional study in patients with neurodegeneration

Direct quantification of CSF α-synuclein by ELISA and first cross-sectional study in patients with neurodegeneration

Because accumulation of α-synuclein (αS) in the brain is a hallmark of Parkinson disease (PD) and related disorders, we examined its occurrence in human cerebrospinal fluid (CSF). Following affinity enrichment and trypsin digestion of CSF collected from a neurologically healthy donor, we identified...

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Veröffentlicht in:Experimental neurology 2008-10, Vol.213 (2), p.315-325
Hauptverfasser: Mollenhauer, Brit, Cullen, Valerie, Kahn, Ilana, Krastins, Bryan, Outeiro, Tiago F., Pepivani, Imelda, Ng, Juliana, Schulz-Schaeffer, Walter, Kretzschmar, Hans A., McLean, Pamela J., Trenkwalder, Claudia, Sarracino, David A., VonSattel, Jean-Paul, Locascio, Joseph J., El-Agnaf, Omar M.A., Schlossmacher, Michael G.
Format: Artikel
Sprache:eng
Schlagworte:
Adult
Aged
Aged, 80 and over
alpha-Synuclein - cerebrospinal fluid
alpha-Synuclein - genetics
Amino Acid Sequence
Animals
Biological and medical sciences
Biomarker
Biomarkers - cerebrospinal fluid
Cells, Cultured
Cerebrospinal fluid
Cross-Sectional Studies
Dementia
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Female
Humans
Male
Mass spectrometry
Medical sciences
Mice
Middle Aged
Molecular Sequence Data
Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis
Nerve Degeneration - cerebrospinal fluid
Nerve Degeneration - genetics
Nerve Degeneration - pathology
Neurology
Parkinsonism
Rats
α-synuclein
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Zusammenfassung:Because accumulation of α-synuclein (αS) in the brain is a hallmark of Parkinson disease (PD) and related disorders, we examined its occurrence in human cerebrospinal fluid (CSF). Following affinity enrichment and trypsin digestion of CSF collected from a neurologically healthy donor, we identified several αS-derived peptides by mass spectrometry. The concentration of αS amounted to
ISSN:0014-4886
1090-2430
DOI:10.1016/j.expneurol.2008.06.004