Fetal hemoglobin: assessment of glycation and acetylation status by electrospray ionization mass spectrometry
Background: Electrospray ionization mass spectrometry (ESI-MS) can be used for the measurement of glycated adult hemoglobin. Here, we describe the evaluation of ESI-MS for measurement of glycated (GHbF) and acetylated (AcHbF) fetal hemoglobin and the identification by mass of different chains of fet...
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| Veröffentlicht in: | Clinical chemistry and laboratory medicine 2008-09, Vol.46 (9), p.1230-1238 |
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| creator | Davison, Andrew S. Green, Brian N. Roberts, Norman B. |
| description | Background: Electrospray ionization mass spectrometry (ESI-MS) can be used for the measurement of glycated adult hemoglobin. Here, we describe the evaluation of ESI-MS for measurement of glycated (GHbF) and acetylated (AcHbF) fetal hemoglobin and the identification by mass of different chains of fetal hemoglobin. Methods: Blood samples were diluted in an acidic denaturing solvent, desalted with AG 50W-X8 resin and introduced directly into the mass spectrometer. Resulting mass spectra were processed to determine the percentage of GHbF and AcHbF and the γ-chain masses. Results: The procedure yielded reproducible quantitative assay of GHbF and AcHbF, with coefficients of variation |
| doi_str_mv | 10.1515/CCLM.2008.257 |
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Here, we describe the evaluation of ESI-MS for measurement of glycated (GHbF) and acetylated (AcHbF) fetal hemoglobin and the identification by mass of different chains of fetal hemoglobin. Methods: Blood samples were diluted in an acidic denaturing solvent, desalted with AG 50W-X8 resin and introduced directly into the mass spectrometer. Resulting mass spectra were processed to determine the percentage of GHbF and AcHbF and the γ-chain masses. Results: The procedure yielded reproducible quantitative assay of GHbF and AcHbF, with coefficients of variation <4.9%. Measurement of α-chain glycation was similarly reproducible and is suggested as an alternative marker of glycemic control. Marked increases in glycation occurred in dried spot blood samples, which were related to duration of storage, temperature and glucose concentration. Molecular masses of fetal hemoglobin chains were also determined and in 42 neonates studied, two types A and B were identified, two-thirds were type A with γ-chain masses corresponding to Gγ and Aγ. In type B, the relative abundance of the Aγ-chain was less and the apparent intensity of the Gγ-chain was higher. Conclusions: ESI-MS can be used for the estimation of GHbF and AcHbF and the accurate measurement of fetal γ-chain masses. The use of whole blood is preferred for analysis. Clin Chem Lab Med 2008;46:1230–8.</description><identifier>ISSN: 1434-6621</identifier><identifier>EISSN: 1437-4331</identifier><identifier>DOI: 10.1515/CCLM.2008.257</identifier><identifier>PMID: 18597590</identifier><language>eng</language><publisher>Berlin: Walter de Gruyter</publisher><subject>acetylated hemoglobin ; Acetylation ; Adult ; Biological and medical sciences ; Blood Chemical Analysis - methods ; Blood Glucose - metabolism ; Female ; Fetal Blood - metabolism ; Fetus - metabolism ; General aspects ; glycated fetal hemoglobin ; Glycated Hemoglobin A - metabolism ; Glycosylation ; Hemoglobins - metabolism ; Humans ; Infant, Newborn ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Reproducibility of Results ; Sensitivity and Specificity ; Specimen Handling ; Spectrometry, Mass, Electrospray Ionization ; α- and γ-chains</subject><ispartof>Clinical chemistry and laboratory medicine, 2008-09, Vol.46 (9), p.1230-1238</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-3d95ae4822fb1cf8b580f2ce43f1f0e867ffa2b96b921e87285a568f96daf1983</citedby><cites>FETCH-LOGICAL-c412t-3d95ae4822fb1cf8b580f2ce43f1f0e867ffa2b96b921e87285a568f96daf1983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.degruyter.com/document/doi/10.1515/CCLM.2008.257/pdf$$EPDF$$P50$$Gwalterdegruyter$$H</linktopdf><linktohtml>$$Uhttps://www.degruyter.com/document/doi/10.1515/CCLM.2008.257/html$$EHTML$$P50$$Gwalterdegruyter$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,66725,68509</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20691728$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18597590$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Davison, Andrew S.</creatorcontrib><creatorcontrib>Green, Brian N.</creatorcontrib><creatorcontrib>Roberts, Norman B.</creatorcontrib><title>Fetal hemoglobin: assessment of glycation and acetylation status by electrospray ionization mass spectrometry</title><title>Clinical chemistry and laboratory medicine</title><addtitle>Clin Chem Lab Med</addtitle><description>Background: Electrospray ionization mass spectrometry (ESI-MS) can be used for the measurement of glycated adult hemoglobin. Here, we describe the evaluation of ESI-MS for measurement of glycated (GHbF) and acetylated (AcHbF) fetal hemoglobin and the identification by mass of different chains of fetal hemoglobin. Methods: Blood samples were diluted in an acidic denaturing solvent, desalted with AG 50W-X8 resin and introduced directly into the mass spectrometer. Resulting mass spectra were processed to determine the percentage of GHbF and AcHbF and the γ-chain masses. Results: The procedure yielded reproducible quantitative assay of GHbF and AcHbF, with coefficients of variation <4.9%. Measurement of α-chain glycation was similarly reproducible and is suggested as an alternative marker of glycemic control. Marked increases in glycation occurred in dried spot blood samples, which were related to duration of storage, temperature and glucose concentration. Molecular masses of fetal hemoglobin chains were also determined and in 42 neonates studied, two types A and B were identified, two-thirds were type A with γ-chain masses corresponding to Gγ and Aγ. In type B, the relative abundance of the Aγ-chain was less and the apparent intensity of the Gγ-chain was higher. Conclusions: ESI-MS can be used for the estimation of GHbF and AcHbF and the accurate measurement of fetal γ-chain masses. The use of whole blood is preferred for analysis. Clin Chem Lab Med 2008;46:1230–8.</description><subject>acetylated hemoglobin</subject><subject>Acetylation</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Blood Chemical Analysis - methods</subject><subject>Blood Glucose - metabolism</subject><subject>Female</subject><subject>Fetal Blood - metabolism</subject><subject>Fetus - metabolism</subject><subject>General aspects</subject><subject>glycated fetal hemoglobin</subject><subject>Glycated Hemoglobin A - metabolism</subject><subject>Glycosylation</subject><subject>Hemoglobins - metabolism</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Specimen Handling</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>α- and γ-chains</subject><issn>1434-6621</issn><issn>1437-4331</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1v1DAURSMEoqWwZIu8gV0GfyY2YoNGTIsYBEhFVGwsx3keUpxksB3R8OvxNKN2w8rPeuddXZ2ieE7wiggiXq_X208rirFcUVE_KE4JZ3XJGSMPb2deVhUlJ8WTGK8xJkLw-nFxQqRQtVD4tOg3kIxHP6Efd35suuENMjFCjD0MCY0O7fxsTerGAZmhRcZCmv3yj8mkKaJmRuDBpjDGfTAzyqvu70L0OQrF_e2yhxTmp8UjZ3yEZ8f3rPi2eX-5vii3n88_rN9tS8sJTSVrlTDAJaWuIdbJRkjsqAXOHHEYZFU7Z2ijqkZRArKmUhhRSaeq1jiiJDsrXi25-zD-niAm3XfRgvdmgHGKulKCY0YOYLmANtePAZzeh643YdYE64NfffCrD3519pv5F8fgqemhvaePQjPw8giYaI13wQy2i3ccxZUiuW_m3i7cH-MThBZ2YZrzoK_HKQzZzf8L8HxOGb7v3cUEN3fxJvzSVc1qob9ecn119eVC_vi40d_ZPxZFqD4</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Davison, Andrew S.</creator><creator>Green, Brian N.</creator><creator>Roberts, Norman B.</creator><general>Walter de Gruyter</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Fetal hemoglobin: assessment of glycation and acetylation status by electrospray ionization mass spectrometry</title><author>Davison, Andrew S. ; Green, Brian N. ; Roberts, Norman B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-3d95ae4822fb1cf8b580f2ce43f1f0e867ffa2b96b921e87285a568f96daf1983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>acetylated hemoglobin</topic><topic>Acetylation</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Blood Chemical Analysis - methods</topic><topic>Blood Glucose - metabolism</topic><topic>Female</topic><topic>Fetal Blood - metabolism</topic><topic>Fetus - metabolism</topic><topic>General aspects</topic><topic>glycated fetal hemoglobin</topic><topic>Glycated Hemoglobin A - metabolism</topic><topic>Glycosylation</topic><topic>Hemoglobins - metabolism</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Specimen Handling</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>α- and γ-chains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Davison, Andrew S.</creatorcontrib><creatorcontrib>Green, Brian N.</creatorcontrib><creatorcontrib>Roberts, Norman B.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry and laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Davison, Andrew S.</au><au>Green, Brian N.</au><au>Roberts, Norman B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fetal hemoglobin: assessment of glycation and acetylation status by electrospray ionization mass spectrometry</atitle><jtitle>Clinical chemistry and laboratory medicine</jtitle><addtitle>Clin Chem Lab Med</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>46</volume><issue>9</issue><spage>1230</spage><epage>1238</epage><pages>1230-1238</pages><issn>1434-6621</issn><eissn>1437-4331</eissn><abstract>Background: Electrospray ionization mass spectrometry (ESI-MS) can be used for the measurement of glycated adult hemoglobin. Here, we describe the evaluation of ESI-MS for measurement of glycated (GHbF) and acetylated (AcHbF) fetal hemoglobin and the identification by mass of different chains of fetal hemoglobin. Methods: Blood samples were diluted in an acidic denaturing solvent, desalted with AG 50W-X8 resin and introduced directly into the mass spectrometer. Resulting mass spectra were processed to determine the percentage of GHbF and AcHbF and the γ-chain masses. Results: The procedure yielded reproducible quantitative assay of GHbF and AcHbF, with coefficients of variation <4.9%. Measurement of α-chain glycation was similarly reproducible and is suggested as an alternative marker of glycemic control. Marked increases in glycation occurred in dried spot blood samples, which were related to duration of storage, temperature and glucose concentration. Molecular masses of fetal hemoglobin chains were also determined and in 42 neonates studied, two types A and B were identified, two-thirds were type A with γ-chain masses corresponding to Gγ and Aγ. In type B, the relative abundance of the Aγ-chain was less and the apparent intensity of the Gγ-chain was higher. Conclusions: ESI-MS can be used for the estimation of GHbF and AcHbF and the accurate measurement of fetal γ-chain masses. The use of whole blood is preferred for analysis. Clin Chem Lab Med 2008;46:1230–8.</abstract><cop>Berlin</cop><cop>New York, NY</cop><pub>Walter de Gruyter</pub><pmid>18597590</pmid><doi>10.1515/CCLM.2008.257</doi><tpages>9</tpages></addata></record> |
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| subjects | acetylated hemoglobin Acetylation Adult Biological and medical sciences Blood Chemical Analysis - methods Blood Glucose - metabolism Female Fetal Blood - metabolism Fetus - metabolism General aspects glycated fetal hemoglobin Glycated Hemoglobin A - metabolism Glycosylation Hemoglobins - metabolism Humans Infant, Newborn Investigative techniques, diagnostic techniques (general aspects) Medical sciences Reproducibility of Results Sensitivity and Specificity Specimen Handling Spectrometry, Mass, Electrospray Ionization α- and γ-chains |
| title | Fetal hemoglobin: assessment of glycation and acetylation status by electrospray ionization mass spectrometry |
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