Fetal hemoglobin: assessment of glycation and acetylation status by electrospray ionization mass spectrometry

Fetal hemoglobin: assessment of glycation and acetylation status by electrospray ionization mass spectrometry

Background: Electrospray ionization mass spectrometry (ESI-MS) can be used for the measurement of glycated adult hemoglobin. Here, we describe the evaluation of ESI-MS for measurement of glycated (GHbF) and acetylated (AcHbF) fetal hemoglobin and the identification by mass of different chains of fet...

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Veröffentlicht in:Clinical chemistry and laboratory medicine 2008-09, Vol.46 (9), p.1230-1238
Hauptverfasser: Davison, Andrew S., Green, Brian N., Roberts, Norman B.
Format: Artikel
Sprache:eng
Schlagworte:
acetylated hemoglobin
Acetylation
Adult
Biological and medical sciences
Blood Chemical Analysis - methods
Blood Glucose - metabolism
Female
Fetal Blood - metabolism
Fetus - metabolism
General aspects
glycated fetal hemoglobin
Glycated Hemoglobin A - metabolism
Glycosylation
Hemoglobins - metabolism
Humans
Infant, Newborn
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Reproducibility of Results
Sensitivity and Specificity
Specimen Handling
Spectrometry, Mass, Electrospray Ionization
α- and γ-chains
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Zusammenfassung:Background: Electrospray ionization mass spectrometry (ESI-MS) can be used for the measurement of glycated adult hemoglobin. Here, we describe the evaluation of ESI-MS for measurement of glycated (GHbF) and acetylated (AcHbF) fetal hemoglobin and the identification by mass of different chains of fetal hemoglobin. Methods: Blood samples were diluted in an acidic denaturing solvent, desalted with AG 50W-X8 resin and introduced directly into the mass spectrometer. Resulting mass spectra were processed to determine the percentage of GHbF and AcHbF and the γ-chain masses. Results: The procedure yielded reproducible quantitative assay of GHbF and AcHbF, with coefficients of variation
ISSN:1434-6621
1437-4331
DOI:10.1515/CCLM.2008.257