SERRS-Based Enzymatic Probes for the Detection of Protease Activity

Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be “switched off” and subsequent exposure to protease restores the SERRS response. The substrate...

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Veröffentlicht in:Journal of the American Chemical Society 2008-09, Vol.130 (36), p.11846-11847
Hauptverfasser: Ingram, Andrew, Byers, Louise, Faulds, Karen, Moore, Barry D, Graham, Duncan
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container_issue 36
container_start_page 11846
container_title Journal of the American Chemical Society
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creator Ingram, Andrew
Byers, Louise
Faulds, Karen
Moore, Barry D
Graham, Duncan
description Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be “switched off” and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml−1.
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subjects Amino Acids - chemistry
Amino Acids - metabolism
Lipase - analysis
Lipase - metabolism
Peptide Hydrolases - analysis
Peptide Hydrolases - metabolism
Spectrum Analysis, Raman - methods
title SERRS-Based Enzymatic Probes for the Detection of Protease Activity
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