The application of CdTe@SiO2 particles in immunoassay

CdTe@SiO(2) fluorescent particles were synthesized via hydrolysis and condensation of tetraethyl orthosilicate (TEOS) in water-in-oil (W/O) emulsion. Uniform luminophore-doped silica nanoparticles with 100nm in diameter were obtained using microemulsion method and characterized by SEM. Antibody prot...

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Veröffentlicht in:	Talanta (Oxford) 2008-09, Vol.76 (5), p.1053-1057
Hauptverfasser:	LI JIN, YU, Dong-Dong, YAN LIU, ZHAO, Xiao-Li, ZHOU, Jian-Guang
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Antigens - immunology Antigens - metabolism Avidin - metabolism Biotinylation Cadmium Compounds - chemical synthesis Cadmium Compounds - chemistry Chemistry Exact sciences and technology Fluorescein-5-isothiocyanate - metabolism Goats - immunology Immunoassay - methods Immunoglobulin G - immunology Immunoglobulin G - metabolism Miscellaneous Nanoparticles - chemistry Silicon Dioxide - chemical synthesis Silicon Dioxide - chemistry Spectrometric and optical methods Spectrometry, Fluorescence Substrate Specificity Tellurium - chemistry
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Beschreibung
Zusammenfassung:	CdTe@SiO(2) fluorescent particles were synthesized via hydrolysis and condensation of tetraethyl orthosilicate (TEOS) in water-in-oil (W/O) emulsion. Uniform luminophore-doped silica nanoparticles with 100nm in diameter were obtained using microemulsion method and characterized by SEM. Antibody proteins were successfully conjugated to the fluorescent particles by the reaction of avidin and biotin, which were confirmed by fluorescence spectra. CdTe@SiO(2) fluorescent particles were potentially useful for the applications in biolabeling and imaging.
ISSN:	0039-9140 1873-3573
DOI:	10.1016/j.talanta.2008.05.002