Target-Catalyzed Transfer Reactions for the Amplified Detection of RNA
Strong signals: The RNA‐catalyzed transfer of a biotin reporter was used to preamplify a signal that was detected in an enzyme‐based readout, thereby increasing its sensitivity. The advantages of combining the covalent attachment of biotin and preamplification were demonstrated by the detection of 5...
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| Veröffentlicht in: | Angewandte Chemie (International ed.) 2008-09, Vol.47 (37), p.7119-7122 |
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| container_issue | 37 |
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| container_title | Angewandte Chemie (International ed.) |
| container_volume | 47 |
| creator | Grossmann, Tom N Röglin, Lars Seitz, Oliver |
| description | Strong signals: The RNA‐catalyzed transfer of a biotin reporter was used to preamplify a signal that was detected in an enzyme‐based readout, thereby increasing its sensitivity. The advantages of combining the covalent attachment of biotin and preamplification were demonstrated by the detection of 500 attomol HIV RNA. The flexibility requirements of DNA‐ and RNA‐catalyzed transfer reactions were tested. |
| doi_str_mv | 10.1002/anie.200801355 |
| format | Article |
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| ispartof | Angewandte Chemie (International ed.), 2008-09, Vol.47 (37), p.7119-7122 |
| issn | 1433-7851 1521-3773 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69486175 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | analytical methods Biotin - chemistry Catalysis Enzyme-Linked Immunosorbent Assay - methods HIV-1 - genetics Molecular Probes - chemistry nucleic acids Peptide Nucleic Acids - chemistry Polymerase Chain Reaction RNA - analysis RNA detection Sensitivity and Specificity signal amplification template catalysis |
| title | Target-Catalyzed Transfer Reactions for the Amplified Detection of RNA |
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