Target-Catalyzed Transfer Reactions for the Amplified Detection of RNA

Target-Catalyzed Transfer Reactions for the Amplified Detection of RNA

Strong signals: The RNA‐catalyzed transfer of a biotin reporter was used to preamplify a signal that was detected in an enzyme‐based readout, thereby increasing its sensitivity. The advantages of combining the covalent attachment of biotin and preamplification were demonstrated by the detection of 5...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Angewandte Chemie (International ed.) 2008-09, Vol.47 (37), p.7119-7122
Hauptverfasser: Grossmann, Tom N, Röglin, Lars, Seitz, Oliver
Format: Artikel
Sprache:eng
Schlagworte:
analytical methods
Biotin - chemistry
Catalysis
Enzyme-Linked Immunosorbent Assay - methods
HIV-1 - genetics
Molecular Probes - chemistry
nucleic acids
Peptide Nucleic Acids - chemistry
Polymerase Chain Reaction
RNA - analysis
RNA detection
Sensitivity and Specificity
signal amplification
template catalysis
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Strong signals: The RNA‐catalyzed transfer of a biotin reporter was used to preamplify a signal that was detected in an enzyme‐based readout, thereby increasing its sensitivity. The advantages of combining the covalent attachment of biotin and preamplification were demonstrated by the detection of 500 attomol HIV RNA. The flexibility requirements of DNA‐ and RNA‐catalyzed transfer reactions were tested.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200801355