Quantification of hepcidin-25 in human serum by isotope dilution micro-HPLC-tandem mass spectrometry

For sample preparation we added 5 µL concentrated formic acid and 50 µL internal standard solution (1450 nmol/L in water, 0.04% acetic acid) to 45 µL human serum or calibrator. Future clinical validation studies will be necessary to evaluate the influence of disease status and the usefulness of hepc...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 2008-09, Vol.54 (9), p.1584-1586
Hauptverfasser:	Kobold, Uwe, Dülffer, Thomas, Dangl, Markus, Escherich, Achim, Kubbies, Manfred, Röddiger, Ralf, Wright, Jo Anne
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetic acid Antimicrobial Cationic Peptides - blood Antimicrobial Cationic Peptides - chemistry Carbon Isotopes Chromatography Chromatography, High Pressure Liquid - methods Chronic illnesses Comparative studies Fasting Female Health Hepcidins Humans Indicator Dilution Techniques Liquid chromatography Male Mass spectrometry Nitrogen Isotopes Sample preparation Tandem Mass Spectrometry - methods Validation studies
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	For sample preparation we added 5 µL concentrated formic acid and 50 µL internal standard solution (1450 nmol/L in water, 0.04% acetic acid) to 45 µL human serum or calibrator. Future clinical validation studies will be necessary to evaluate the influence of disease status and the usefulness of hepcidin measurement in differential diagnoses.
ISSN:	0009-9147 1530-8561
DOI:	10.1373/clinchem.2008.107029