Sample preparation method for plasma membrane proteome analysis

The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The de...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-09, Vol.872 (1), p.177-180
Hauptverfasser:	Park, Seung-Ah, Kim, Mi-Ryung, Kim, Pan-Kyeom, Cho, Hyung-Don, Han, Gi-Yeon, Kim, Chan-Wha
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Blood Proteins - analysis Blotting, Western Electrophoresis, Gel, Two-Dimensional Fundamental and applied biological sciences. Psychology General pharmacology hMSCs Medical sciences Percoll Pharmacology. Drug treatments Plasma membrane Proteome Two-dimensional electrophoresis Two-phase extraction Ultracentrifugation
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Beschreibung
Zusammenfassung:	The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2008.07.028