Photoreactivity of 5-iodouracil-containing DNA-Sso7d complex

X-ray structure of DNA-Sso7d complex indicated that binding of this protein causes sharp DNA bending. In order to examine whether this protein also causes DNA bending in solution, photoreactions of 1U-substituted DNA in the presence and the absence of Sso7d protein were investigated. It was found th...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nucleic acids symposium series (1979) 1999 (42), p.171-172
Hauptverfasser:	Oyoshi, T, Sugiyama, H, Wang, A H
Format:	Artikel
Sprache:	eng
Schlagworte:	Archaeal Proteins - chemistry Base Sequence Crystallography, X-Ray DNA - chemistry DNA-Binding Proteins - chemistry Nucleic Acid Conformation Oligodeoxyribonucleotides - chemistry Photochemistry Uracil - analogs & derivatives
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	X-ray structure of DNA-Sso7d complex indicated that binding of this protein causes sharp DNA bending. In order to examine whether this protein also causes DNA bending in solution, photoreactions of 1U-substituted DNA in the presence and the absence of Sso7d protein were investigated. It was found that the unusual intrastrand hydrogen abstraction at methyl of adjacent thymine occurred efficiently at the observed bending site of crystal structure.
ISSN:	0261-3166