High-performance liquid chromatographic analysis of new triazole antifungal agent SYN-2869 and its derivatives in plasma
A simple reversed-phase high-performance liquid chromatography (HPLC) method with UV detection was developed and validated for the quantitation of SYN-2869, a novel triazole antifungal agent and its analogs in rat plasma. The method involved a simple precipitation of plasma protein with acetonitrile...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 1999-09, Vol.20 (5), p.791-797 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Khan, Jehangir K Montaseri, Hashem Poglod, Marzena Bu, Hai-Zhi Daneshtalab, Mohsen Micetich, Ronald G |
| description | A simple reversed-phase high-performance liquid chromatography (HPLC) method with UV detection was developed and validated for the quantitation of SYN-2869, a novel triazole antifungal agent and its analogs in rat plasma. The method involved a simple precipitation of plasma protein with acetonitrile (1:10 ratio). The reconstituted sample after evaporation to dryness was injected onto a HPLC column. SYN-2869 and its analogs were separated from the matrix components on a symmetry C18 column using an aqueous mobile phase of acetonitrile and water with a flow rate of 1 ml min
−1. A step gradient of 40–80% acetonitrile eluted all four compounds. The run time was 30 min. The linear range was 0.5–10 μg ml
−1 (
r
2>0.999). The limit of quantitation was 0.5 μg ml
−1. The inter-day precision and accuracy for SYN-2869 standard concentration were from 1.9 to 8.5% and from −1.4 to +4.4%, respectively. The precision and accuracy of intra-day quality control samples were from 4.6 to 5.2% and from 4.6 to 12%, respectively. |
| doi_str_mv | 10.1016/S0731-7085(99)00080-1 |
| format | Article |
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−1. A step gradient of 40–80% acetonitrile eluted all four compounds. The run time was 30 min. The linear range was 0.5–10 μg ml
−1 (
r
2>0.999). The limit of quantitation was 0.5 μg ml
−1. The inter-day precision and accuracy for SYN-2869 standard concentration were from 1.9 to 8.5% and from −1.4 to +4.4%, respectively. The precision and accuracy of intra-day quality control samples were from 4.6 to 5.2% and from 4.6 to 12%, respectively.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(99)00080-1</identifier><identifier>PMID: 10701987</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Animals ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antifungal agents ; Antifungal Agents - analysis ; Antifungal Agents - blood ; Antifungals ; Azoles ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; General pharmacology ; Medical sciences ; Mice ; Molecular Structure ; Pharmacology. Drug treatments ; Piperazines - analysis ; Piperazines - blood ; Piperazines - pharmacokinetics ; Rats ; Reproducibility of Results ; Triazoles - analysis ; Triazoles - blood ; Triazoles - pharmacokinetics</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 1999-09, Vol.20 (5), p.791-797</ispartof><rights>1999 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-187c9b763f09402d1741eb12d839088067c9bcfedb49be994a6d8c83c5ef7163</citedby><cites>FETCH-LOGICAL-c390t-187c9b763f09402d1741eb12d839088067c9bcfedb49be994a6d8c83c5ef7163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708599000801$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1909657$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10701987$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khan, Jehangir K</creatorcontrib><creatorcontrib>Montaseri, Hashem</creatorcontrib><creatorcontrib>Poglod, Marzena</creatorcontrib><creatorcontrib>Bu, Hai-Zhi</creatorcontrib><creatorcontrib>Daneshtalab, Mohsen</creatorcontrib><creatorcontrib>Micetich, Ronald G</creatorcontrib><title>High-performance liquid chromatographic analysis of new triazole antifungal agent SYN-2869 and its derivatives in plasma</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A simple reversed-phase high-performance liquid chromatography (HPLC) method with UV detection was developed and validated for the quantitation of SYN-2869, a novel triazole antifungal agent and its analogs in rat plasma. The method involved a simple precipitation of plasma protein with acetonitrile (1:10 ratio). The reconstituted sample after evaporation to dryness was injected onto a HPLC column. SYN-2869 and its analogs were separated from the matrix components on a symmetry C18 column using an aqueous mobile phase of acetonitrile and water with a flow rate of 1 ml min
−1. A step gradient of 40–80% acetonitrile eluted all four compounds. The run time was 30 min. The linear range was 0.5–10 μg ml
−1 (
r
2>0.999). The limit of quantitation was 0.5 μg ml
−1. The inter-day precision and accuracy for SYN-2869 standard concentration were from 1.9 to 8.5% and from −1.4 to +4.4%, respectively. The precision and accuracy of intra-day quality control samples were from 4.6 to 5.2% and from 4.6 to 12%, respectively.</description><subject>Analysis</subject><subject>Animals</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antifungal agents</subject><subject>Antifungal Agents - analysis</subject><subject>Antifungal Agents - blood</subject><subject>Antifungals</subject><subject>Azoles</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>General pharmacology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Molecular Structure</subject><subject>Pharmacology. Drug treatments</subject><subject>Piperazines - analysis</subject><subject>Piperazines - blood</subject><subject>Piperazines - pharmacokinetics</subject><subject>Rats</subject><subject>Reproducibility of Results</subject><subject>Triazoles - analysis</subject><subject>Triazoles - blood</subject><subject>Triazoles - pharmacokinetics</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vFCEYh4nR2G31I2g4GFMPo7Azw5-TMY1ak0YP7UFPhIGXXQwzTIFZrZ9etrtRb57ew-_5wcsDQs8oeU0JZW-uCW9pw4noz6V8RQgRpKEP0IoK3jZr1n19iFZ_kBN0mvP3CvVUdo_RCSWcUCn4Cv289JttM0NyMY16MoCDv128xWab4qhL3CQ9b73BetLhLvuMo8MT_MAlef0rBqhB8W6ZNjpgvYGp4Otvn5u1YLImFvuSsYXkd7r4HWTsJzwHnUf9BD1yOmR4epxn6ObD-5uLy-bqy8dPF--uGtNKUpr6HCMHzlpHZEfWlvKOwkDXVtRYCML2sXFgh04OIGWnmRVGtKYHxylrz9DLw7FzircL5KJGnw2EoCeIS1ZMdp0kXFawP4AmxZwTODUnP-p0pyhRe-Pq3rja61RSqnvjitbe8-MFyzCC_ad1UFyBF0dAZ6ODS9Wyz385SSTr99jbAwZVxs5DUtl4qB9ifQJTlI3-P5v8BsHWnjs</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>Khan, Jehangir K</creator><creator>Montaseri, Hashem</creator><creator>Poglod, Marzena</creator><creator>Bu, Hai-Zhi</creator><creator>Daneshtalab, Mohsen</creator><creator>Micetich, Ronald G</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990901</creationdate><title>High-performance liquid chromatographic analysis of new triazole antifungal agent SYN-2869 and its derivatives in plasma</title><author>Khan, Jehangir K ; Montaseri, Hashem ; Poglod, Marzena ; Bu, Hai-Zhi ; Daneshtalab, Mohsen ; Micetich, Ronald G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-187c9b763f09402d1741eb12d839088067c9bcfedb49be994a6d8c83c5ef7163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antifungal agents</topic><topic>Antifungal Agents - analysis</topic><topic>Antifungal Agents - blood</topic><topic>Antifungals</topic><topic>Azoles</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>General pharmacology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Molecular Structure</topic><topic>Pharmacology. Drug treatments</topic><topic>Piperazines - analysis</topic><topic>Piperazines - blood</topic><topic>Piperazines - pharmacokinetics</topic><topic>Rats</topic><topic>Reproducibility of Results</topic><topic>Triazoles - analysis</topic><topic>Triazoles - blood</topic><topic>Triazoles - pharmacokinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Jehangir K</creatorcontrib><creatorcontrib>Montaseri, Hashem</creatorcontrib><creatorcontrib>Poglod, Marzena</creatorcontrib><creatorcontrib>Bu, Hai-Zhi</creatorcontrib><creatorcontrib>Daneshtalab, Mohsen</creatorcontrib><creatorcontrib>Micetich, Ronald G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Jehangir K</au><au>Montaseri, Hashem</au><au>Poglod, Marzena</au><au>Bu, Hai-Zhi</au><au>Daneshtalab, Mohsen</au><au>Micetich, Ronald G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-performance liquid chromatographic analysis of new triazole antifungal agent SYN-2869 and its derivatives in plasma</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>20</volume><issue>5</issue><spage>791</spage><epage>797</epage><pages>791-797</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A simple reversed-phase high-performance liquid chromatography (HPLC) method with UV detection was developed and validated for the quantitation of SYN-2869, a novel triazole antifungal agent and its analogs in rat plasma. The method involved a simple precipitation of plasma protein with acetonitrile (1:10 ratio). The reconstituted sample after evaporation to dryness was injected onto a HPLC column. SYN-2869 and its analogs were separated from the matrix components on a symmetry C18 column using an aqueous mobile phase of acetonitrile and water with a flow rate of 1 ml min
−1. A step gradient of 40–80% acetonitrile eluted all four compounds. The run time was 30 min. The linear range was 0.5–10 μg ml
−1 (
r
2>0.999). The limit of quantitation was 0.5 μg ml
−1. The inter-day precision and accuracy for SYN-2869 standard concentration were from 1.9 to 8.5% and from −1.4 to +4.4%, respectively. The precision and accuracy of intra-day quality control samples were from 4.6 to 5.2% and from 4.6 to 12%, respectively.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10701987</pmid><doi>10.1016/S0731-7085(99)00080-1</doi><tpages>7</tpages></addata></record> |
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| subjects | Analysis Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antifungal agents Antifungal Agents - analysis Antifungal Agents - blood Antifungals Azoles Biological and medical sciences Chromatography, High Pressure Liquid General pharmacology Medical sciences Mice Molecular Structure Pharmacology. Drug treatments Piperazines - analysis Piperazines - blood Piperazines - pharmacokinetics Rats Reproducibility of Results Triazoles - analysis Triazoles - blood Triazoles - pharmacokinetics |
| title | High-performance liquid chromatographic analysis of new triazole antifungal agent SYN-2869 and its derivatives in plasma |
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