High-performance liquid chromatographic analysis of new triazole antifungal agent SYN-2869 and its derivatives in plasma

A simple reversed-phase high-performance liquid chromatography (HPLC) method with UV detection was developed and validated for the quantitation of SYN-2869, a novel triazole antifungal agent and its analogs in rat plasma. The method involved a simple precipitation of plasma protein with acetonitrile (1:10 ratio). The reconstituted sample after evaporation to dryness was injected onto a HPLC column. SYN-2869 and its analogs were separated from the matrix components on a symmetry C18 column using an aqueous mobile phase of acetonitrile and water with a flow rate of 1 ml min −1. A step gradient of 40–80% acetonitrile eluted all four compounds. The run time was 30 min. The linear range was 0.5–10 μg ml −1 ( r 2>0.999). The limit of quantitation was 0.5 μg ml −1. The inter-day precision and accuracy for SYN-2869 standard concentration were from 1.9 to 8.5% and from −1.4 to +4.4%, respectively. The precision and accuracy of intra-day quality control samples were from 4.6 to 5.2% and from 4.6 to 12%, respectively.