Peptide mass fingerprinting using isotopically encoded photo-crosslinking amino acids

When isotopically labelled photo-crosslinking amino acids are site-specifically incorporated into proteins, in combination with the corresponding non-labeled analogue, cross-linked tryptic peptides are easily identified in mass spectra via characteristic "doublet" patterns.

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Bibliographische Detailangaben
Veröffentlicht in:	Molecular bioSystems 2008-09, Vol.4 (9), p.934-936
Hauptverfasser:	Wilkins, Bryan J, Daggett, Kelly A, Cropp, T Ashton
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - chemistry Amino Acids - metabolism Cross-Linking Reagents - chemistry Isotope Labeling Mass Spectrometry Models, Molecular Molecular Weight Peptide Fragments - chemistry Peptide Fragments - metabolism Peptide Mapping - methods
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	When isotopically labelled photo-crosslinking amino acids are site-specifically incorporated into proteins, in combination with the corresponding non-labeled analogue, cross-linked tryptic peptides are easily identified in mass spectra via characteristic "doublet" patterns.
ISSN:	1742-2051
DOI:	10.1039/b801512k