Real-Time Detection of Protein-Water Dynamics upon Protein Folding by Terahertz Absorption Spectroscopy

Real-Time Detection of Protein-Water Dynamics upon Protein Folding by Terahertz Absorption Spectroscopy

Take a look around: Kinetic terahertz absorption (KITA) spectroscopy has been used to monitor the attenuation and phase shift of a terahertz electric field transmitted through a protein during refolding. Comparison with data from fluorescence, circular dichroism, and small angle X‐ray scattering sho...

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Veröffentlicht in:Angewandte Chemie International Edition 2008-08, Vol.47 (34), p.6486-6489
Hauptverfasser: Kim, Seung Joong, Born, Benjamin, Havenith, Martina, Gruebele, Martin
Format: Artikel
Sprache:eng
Schlagworte:
Absorption
circular dichroism
Electricity
fluorescence
Protein Folding
secondary structure
Spectrum Analysis - methods
terahertz spectroscopy
Time Factors
Ubiquitin - chemistry
Water - chemistry
X-Ray Diffraction
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Zusammenfassung:Take a look around: Kinetic terahertz absorption (KITA) spectroscopy has been used to monitor the attenuation and phase shift of a terahertz electric field transmitted through a protein during refolding. Comparison with data from fluorescence, circular dichroism, and small angle X‐ray scattering shows that KITA monitors rearrangements of the hydration water–protein interactions during early collapse and secondary structure formation.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200802281