Introduction of a real-time based blood group genotyping approach

Background and Objective Genotyping may be applied for rare blood group polymorphisms in a high‐throughput mode as well for the molecular determination of blood groups due to unclear serological results. Material and Methods We developed and validated a DNA typing method for the determination of KEL1/2, JK1/2, FY1/2, FY0, MNS1/2, MNS3/4, DO1/2, CO1/2 and LU1/2 alleles using a melting curve analysis downstream from a fully automated DNA extraction. All assays were validated in terms of specificity, sensitivity, assay variability and robustness. The usability was proven by a batch of 200 blood samples with partially known phenotype. Results Assays for all blood groups were within the range of specificity (100%), assay variability and robustness (coefficient of variance