Localization of the ATP-sensitive potassium channel subunit (Kir6. 1/uK(ATP)-1) in rat brain

The Kir6.1/uK(ATP)-1, subunit of ATP-sensitive K(+) channels (K(ATP)), was localized in adult rat brain by in situ hybridization and immunohistochemistry. The mRNA of this subunit was ubiquitously expressed in various neurons and nuclei of the adult rat brain. Interestingly, Kir6.1/uK(ATP)-1 mRNA wa...

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Veröffentlicht in:	Brain research. Molecular brain research. 1999-12, Vol.74 (1-2), p.15-25
Hauptverfasser:	Zhou, M, Tanaka, O, Sekiguchi, M, Sakabe, K, Anzai, M, Izumida, I, Inoue, T, Kawahara, K, Abe, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - physiology Animals Brain - metabolism Brain - ultrastructure Brain Chemistry Cerebellum - chemistry Cerebellum - metabolism Cerebellum - ultrastructure Diencephalon - chemistry Diencephalon - metabolism Immunohistochemistry In Situ Hybridization Male Medulla Oblongata - chemistry Medulla Oblongata - metabolism Mesencephalon - chemistry Mesencephalon - metabolism Microscopy, Immunoelectron Mitochondria - chemistry Mitochondria - ultrastructure Olfactory Bulb - chemistry Olfactory Bulb - metabolism Pons - chemistry Pons - metabolism Potassium Channels - genetics Potassium Channels - metabolism Rats Rats, Wistar RNA, Messenger - genetics RNA, Messenger - metabolism
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Zusammenfassung:	The Kir6.1/uK(ATP)-1, subunit of ATP-sensitive K(+) channels (K(ATP)), was localized in adult rat brain by in situ hybridization and immunohistochemistry. The mRNA of this subunit was ubiquitously expressed in various neurons and nuclei of the adult rat brain. Interestingly, Kir6.1/uK(ATP)-1 mRNA was also expressed in glial cells. Kir6.1/uK(ATP)-1 protein staining gave a dispersed array of fine dots throughout all neurons and glial cells examined. Under electron microscope, the immunoreactive products were specifically restricted to the mitochondria. The present study indicates that this K(ATP) subunit is localized in the mitochondria and may play a fundamental role in vital brain function.
ISSN:	0169-328X
DOI:	10.1016/S0169-328X(99)00232-6