Zone Electrophoresis in an Inner-cooling Wide-bore Electrophoresis System with UV Detection

A novel, high-performance wide-bore electrophoresis (WE) system with inner-cooling has been developed. By introducing the mode of a shell and tube heat exchanger into this system to remove Joule heat generated during electrophoresis, it is feasible to extend electrophoresis from the conventional capillary (i.d. 1000 µm). The wide tube allows the loading of over 1.0 µL of the sample with an LOD of 3.0 × 10-4 mg/mL (signal-to-noise ratio, 3:1). Satisfactory separations of model compounds have been achieved on the WE system.