Female tick Hyalomma marginatum marginatum salivary glands: preliminary study on protein changes during feeding process and antigens recognized by repeatedly infested cattle

Proteins extracted from salivary glands of unfed, three days and five days fed adult Hyalomma marginatum marginatum were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). We have noticed changes during the three feeding steps. Some proteins disappeared during feeding process (23, 38, 39, 40 to 50, 95 and 112 kDa), they might be proteins which were converted in other substances and are secreted. Other antigens (13 to 14, 20, 25, 29, 165 and 210 kDa) were synthesized as a result of tick attachment and feeding. They may be related to growth and development or are the ciment which fixed the adult. Also, three Holstein calves were infested five times with 100 pairs of adult ticks of the same species. The five infestations were performed two weeks from the previous infestation. The sera before infestations and after each infestation were used in western-blot analyses to identify antigens from five days salivary gland extracts of the primary infestation of ticks. Three antigens (18.7, 50 and 80 kDa) were revealed weakly after the first and the second infestations by sera samples but not at infestation onward. Others (13.5, 17 to 18.5, 25, 30, 70, 133, 176 and 193 kDa) were revealed only by sera taken after manifestation of resistance (third infestation). A 13.5 kDa antigen was particularly revealed when resistance had appeared and became more evident after the fourth and fifth infestations. The late antigens recognized might be associated with establishment of calves resistance against ticks. Des extraits de glandes salivaires de tiques adultes Hyalomma marginatum marginatum ont été analysés par SDS-PAGE. Trois stades de tiques ont été utilisés, des tiques à jeun ou des tiques après trois ou cinq jours de gorgement. Certains antigènes (23, 38, 39, 40 à 50, 95 et 112 kDa) disparaissent tôt durant le processus de gorgement. Ces antigènes sont probablement convertis et sécrétés en d'autres substances. D'autres antigènes (13 à 14, 20, 25, 29, 165 et 210 kDa) sont synthétisés après fixation de la tique et le début du gorgement. Ces antigènes doivent êtres liés à la croissance et au développement de la tique ou aux mécanismes de fixations de cette dernière. En outre, trois bovins de race Holstein ont fait l'objet de cinq infestations massives 1100 paires de tiques par infestation) à intervalles de 15 jours et cela après gorgement de la dernière tique de I'infestation précédente. L'extrait des glandes salivaires des tiques de la première