Morphologically normal, CD30-negative B-lymphocytes with chromosome aberrations in classical Hodgkin's disease: the progenitor cell of the malignant clone?

A recent study observed that numerical chromosome abnormalities in Hodgkin's disease (HD) are detected not only in morphologically abnormal Hodgkin/Reed–Sternberg cells, but also in a fraction of morphologically normal cells. However, the phenotypic constitution of these genetically abnormal, morphologically normal cells and their relationship to the malignant Hodgkin/Reed–Sternberg cells could not be established in the earlier cases studied, because of the low frequency of these cells. The present study investigated two cases of classical Hodgkin's disease containing a relatively large population of such apparently normal cells with aberrant chromosome copy numbers. The phenotype and their position within the developmental route of the malignant compartment were examined by a combined in situ hybridization and immunocytochemistry approach. Numerical abnormalities for chromosome 1 in one case and for chromosomes X, Y, and 1 in the other case were observed not only in CD30‐positive Hodgkin/Reed–Sternberg cells, but also in CD30‐negative, morphologically normal cells. It was shown that these genetically aberrant cells expressed the B‐cell antigen CD19, thus confirming their B‐cell nature. These studies indicate a relationship between the genome aberrations in these genetically abnormal, morphologically normal B‐cells and the Hodgkin/Reed–Sternberg cells, suggesting that they are progenitor cells of the malignant cell fraction. Copyright © 1999 John Wiley & Sons, Ltd.