High-performance liquid chromatography separation methods for the analysis of peptide nucleic acids

An analytical analysis of peptide nucleic acids (PNAs) was carried out by reversed-phase HPLC using a solvent system comprised of aqueous trifluoroacetic acid and acetonitrile. A regression equation was obtained which represents the relationship of the molecular mass, sequence composition and retent...

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Veröffentlicht in:	Journal of Chromatography A 1999-12, Vol.864 (1), p.49-57
Hauptverfasser:	Wei, Yingning, Marino, Michael, Thompson, Barry, Girard, James E
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetonitriles Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Base Composition Base Sequence Biological and medical sciences Chromatography, High Pressure Liquid - methods Drug Stability Fundamental and applied biological sciences. Psychology Molecular Weight Oligonucleotides Peptide nucleic acids Peptide Nucleic Acids - analysis Peptide Nucleic Acids - chemistry Proteins Solvents Trifluoroacetic Acid
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Beschreibung
Zusammenfassung:	An analytical analysis of peptide nucleic acids (PNAs) was carried out by reversed-phase HPLC using a solvent system comprised of aqueous trifluoroacetic acid and acetonitrile. A regression equation was obtained which represents the relationship of the molecular mass, sequence composition and retention time. This equation can be used to estimate the retention time of a known PNA under certain HPLC conditions. In addition to this equation, new HPLC conditions were also optimized which can be used for separation of pure PNAs.
ISSN:	0021-9673
DOI:	10.1016/S0021-9673(99)01013-4