Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology

We have developed a Linnik-type interference microscope provided with a low-coherent light source to obtain topographic images of an intact cellular membrane on a nanometer scale. Our technique is based on measurement of the interference between light reflected from the cell surface and a reference...

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Veröffentlicht in:	Optics express 2008-08, Vol.16 (16), p.12227-12238
Hauptverfasser:	Yamauchi, Toyohiko, Iwai, Hidenao, Miwa, Mitsuharu, Yamashita, Yutaka
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Membrane - ultrastructure Cell Size Equipment Design Equipment Failure Analysis Image Enhancement - instrumentation Interferometry - instrumentation Interferometry - methods Microscopy, Phase-Contrast - instrumentation Microscopy, Phase-Contrast - methods Nanotechnology - instrumentation Sensitivity and Specificity Tomography, Optical Coherence - instrumentation Tomography, Optical Coherence - methods
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Beschreibung
Zusammenfassung:	We have developed a Linnik-type interference microscope provided with a low-coherent light source to obtain topographic images of an intact cellular membrane on a nanometer scale. Our technique is based on measurement of the interference between light reflected from the cell surface and a reference beam. The results show full field surface topography of cultured cells and reveal an intrinsic membrane motion of tens of nanometers.
ISSN:	1094-4087 1094-4087
DOI:	10.1364/oe.16.012227