Gossypol Induced Apoptosis in the Human Promyelocytic Leukemia Cell Line HL 60

In human promyelocytic leukemia cell line HL60, apoptosis was induced by treatment with gossypol that is an inhibitor of protein kinase C. Gossypol acetic acid was added to HL 60 cells at 50, 100, 150 and 200 μM concentrations for six hours. Morphological features of apoptosis as well as internucleo...

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Veröffentlicht in:	The Tohoku Journal of Experimental Medicine 1999, Vol.189(1), pp.51-57
Hauptverfasser:	Balci, Ayse, Sahin, Feride Iffet, Ekmekci, Abdullah
Format:	Artikel
Sprache:	eng
Schlagworte:	apoptosis Apoptosis - drug effects DNA Fragmentation Electrophoresis, Agar Gel Enzyme Inhibitors - pharmacology gossypol Gossypol - pharmacology HL-60 Cells Humans Leukemia, Promyelocytic, Acute protein kinase Protein Kinase C - antagonists & inhibitors Spectrometry, Fluorescence
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Zusammenfassung:	In human promyelocytic leukemia cell line HL60, apoptosis was induced by treatment with gossypol that is an inhibitor of protein kinase C. Gossypol acetic acid was added to HL 60 cells at 50, 100, 150 and 200 μM concentrations for six hours. Morphological features of apoptosis as well as internucleosomal DNA fragmentation were evaluated by light microscope, agarose gel electrophoresis and spectrofluorometric quantitation. Our results indicated that with the effective concentrations of gossypol (50 and 100 μM), apoptosis was induced in HL 60 cells.
ISSN:	0040-8727 1349-3329
DOI:	10.1620/tjem.189.51