High-Resolution Capillary Isoelectric Focusing of Complex Protein Mixtures from Lysates of Microorganisms

High-resolution capillary isoelectric focusing separations of complex protein mixtures have been obtained for cellular lysates of Saccharomyces cerevisiae, Eschericia coli, and Deinococcus radiodurans. High quality separations are shown to be achievable for total protein concentrations of

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Bibliographische Detailangaben
Veröffentlicht in:	Analytical chemistry (Washington) 1999-12, Vol.71 (23), p.5348-5353
Hauptverfasser:	Shen, Yufeng, Xiang, Fan, Veenstra, Timothy D, Fung, Eliza N, Smith, Richard D
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Bacterial Proteins - isolation & purification Biological and medical sciences Blood vessels Electrophoresis, Capillary - methods Escherichia coli - chemistry Fundamental and applied biological sciences. Psychology Fungal Proteins - isolation & purification General aspects, investigation methods Gram-Positive Cocci - chemistry Isoelectric Focusing - methods Microorganisms Proteins Reproducibility of Results Saccharomyces cerevisiae - chemistry
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Beschreibung
Zusammenfassung:	High-resolution capillary isoelectric focusing separations of complex protein mixtures have been obtained for cellular lysates of Saccharomyces cerevisiae, Eschericia coli, and Deinococcus radiodurans. High quality separations are shown to be achievable for total protein concentrations of
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac9909305