Validation and Standardization of Virus Neutralizing Test Using Indirect Immunoperoxidase Technique for the Quantification of Antibodies to Rabies Virus

Summary A virus neutralizing test using an indirect immunoperoxidase technique (VNT‐IIP) for rabies has been developed for the titration of dog and cat serum samples in Japan. The VNT‐IIP has the advantage that results obtained can be viewed by the naked eye. The purpose of this study was to validate the VNT‐IIP and compare it with one of the international standard methods, the fluorescent antibody virus neutralization test (FAVNT). The VNT‐IIP showed satisfactory repeatability, high analytical specificity and good accuracy. Regarding the comparison between the VNT‐IIP and the FAVNT, the VNT‐IIP showed good agreement (91.9%), high sensitivity (92.8%) as well as specificity (87.0%) and good correlation (r = 0.92). As described above, the validation of the VNT‐IIP was satisfactory and the performances of the test proved to be equivalent to those of an international standard method.