Gene transduction by sonoporation

Gene transduction by sonoporation

Gene transduction technologies are essential tools for understanding of gene functions or gene cascades underlying embryogenesis. In this review, we introduce a gene transduction method using microbubble and ultrasound (hereafter referred to as sonoporation). Sonoporation is carried out with relativ...

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Veröffentlicht in:Development, growth & differentiation growth & differentiation, 2008-08, Vol.50 (6), p.517-520
Hauptverfasser: Ohta, Sho, Suzuki, Kentaro, Ogino, Yukiko, Miyagawa, Shinichi, Murashima, Aki, Matsumaru, Daisuke, Yamada, Gen
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Chick Embryo
Electroporation - methods
Gene transduction chick
Gene Transfer Techniques
Green Fluorescent Proteins - metabolism
limb bud
Limb Buds - embryology
Mesoderm - cytology
microbubble
Microbubbles
Models, Genetic
Sonication
sonoporation
Transduction, Genetic
ultrasonics
ultrasound
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Beschreibung
Zusammenfassung:Gene transduction technologies are essential tools for understanding of gene functions or gene cascades underlying embryogenesis. In this review, we introduce a gene transduction method using microbubble and ultrasound (hereafter referred to as sonoporation). Sonoporation is carried out with relatively simple procedures and easily transduces genes into mesenchymal cells without significant damage to target tissues. Therefore, sonoporation is effective for gene transduction to study the molecular mechanisms of morphogenesis.
ISSN:0012-1592
1440-169X
DOI:10.1111/j.1440-169x.2008.01026.x