Membrane Chromatography: Preparation and Applications to Protein Separation
As a result of the convective flow of solutes through porous membranes, membrane chromatography has a higher capture efficiency and a higher productivity than column chromatography and shows most promising industrial applications for the recovery, isolation, and purification of proteins and enzymes....
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Veröffentlicht in: | Biotechnology progress 1999-11, Vol.15 (6), p.1003-1019 |
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creator | Zeng, Xianfang Ruckenstein, Eli |
description | As a result of the convective flow of solutes through porous membranes, membrane chromatography has a higher capture efficiency and a higher productivity than column chromatography and shows most promising industrial applications for the recovery, isolation, and purification of proteins and enzymes. This paper presents a comprehensive review of the methods for preparation of adsorptive membranes (such as surface modification, in situ copolymerization, direct formation from hydrophilic materials, and functionalized particulate‐entrapped membranes) and deals particularly with novel macroporous chitin and chitosan membranes for protein separations developed by the authors. |
doi_str_mv | 10.1021/bp990120e |
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subjects | Biological and medical sciences Biotechnology Carbohydrate Sequence Cellulose - chemistry Chitin - analogs & derivatives Chitin - chemistry Chitosan Chromatography - methods Enzymes - isolation & purification Fundamental and applied biological sciences. Psychology Membranes, Artificial Methods. Procedures. Technologies Molecular Sequence Data Others Protein separation Proteins - isolation & purification Various methods and equipments |
title | Membrane Chromatography: Preparation and Applications to Protein Separation |
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