Mapping a neutralizing epitope on the coat protein of striped jack nervous necrosis virus

Faculty of Applied Biological Science, Hiroshima University, Higashi-hiroshima 739-8528, Japan 1 Author for correspondence: Toyohiko Nishizawa.Fax +81 824 22 7059. e-mail jjnishi{at}ipc.hiroshima-u.ac.jp Striped jack nervous necrosis virus (SJNNV), a fish nodavirus, is the causative agent of viral nervous necrosis in marine fishes. The fish nodaviruses are divided into four different genotypes based on the nucleotide sequence of the coat protein gene. In the present study, partial coat protein genes of fish nodaviruses were expressed. This allowed the serological relationship among the different virus genotypes to be analysed and neutralizing epitopes on the coat protein to be mapped. Western blot analysis revealed that SJNNV and other fish nodavirus genotypes shared a significant number of common antigenic determinants, although SJNNV was serologically distinguishable. The results suggested that the SJNNV determinant for neutralizing MAbs was a linear epitope, which consisted of a repeated amino acid sequence within the coat protein. One of the neutralizing epitopes of SJNNV was deduced to be PAN at aa 254–256 in the coat protein.