Effects of N-methyl-D-aspartate on luteinizing hormone release and Fos-like immunoreactivity in the male White-crowned sparrow (Zonotrichia leucophrys gambelii)

Seasonal breeding is terminated in the White-crowned sparrow by the onset of absolute photorefractoriness, a condition in which the reproductive system is switched off indefinitely until it is dissipated by short day lengths. Absolute photorefractoriness is controlled by the central nervous system; however, the mechanisms underlying GnRH quiescence in photorefractory birds have yet to be elucidated. Using the excitatory amino acid glutamate agonist N-methyl-D-aspartate (NMDA), plasma LH levels in White-crowned sparrows were significantly elevated regardless of the reproductive or photoperiodic condition. NMDA also significantly induced Fos-like immunoreactivity (FLI) within the infundibular nucleus and median eminence, regions previously shown to express FLI after a photoperiodically driven LH rise. NMDA did not induce FLI within GnRH I neurons; instead, it significantly activated cells within the organum vasculosum of the lamina terminalis in close proximity to GnRH I perikarya. These findings provide the first evidence that photorefractoriness is not due to depletion of GnRH stores, as LH and presumably GnRH were secreted in response to excitatory amino acid stimulation. NMDA activation of FLI in the region of the organum vasculosum of the lamina terminalis and the basal tuberal hypothalamus suggests that seasonal reproductive neuroendocrine control may be mediated via cells in the region of the GnRH I perikarya and terminals.