Screen-printed electrodes based on carbon nanotubes and cytochrome P450scc for highly sensitive cholesterol biosensors

This paper is concerned with an investigation of electron transfer between cytochrome P450scc (CYP11A1) immobilized on nanostructured rhodium–graphite electrodes. Multi-walled carbon nanotubes (MWCNT) were deposited onto the rhodium–graphite electrodes by drop casting. Cytochrome P450scc was deposit...

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Veröffentlicht in:Biosensors & bioelectronics 2008-09, Vol.24 (1), p.148-150
Hauptverfasser: Carrara, Sandro, Shumyantseva, Victoria V., Archakov, Alexander I., Samorì, Bruno
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container_issue 1
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container_title Biosensors & bioelectronics
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creator Carrara, Sandro
Shumyantseva, Victoria V.
Archakov, Alexander I.
Samorì, Bruno
description This paper is concerned with an investigation of electron transfer between cytochrome P450scc (CYP11A1) immobilized on nanostructured rhodium–graphite electrodes. Multi-walled carbon nanotubes (MWCNT) were deposited onto the rhodium–graphite electrodes by drop casting. Cytochrome P450scc was deposited onto MWCNT-modified rhodium–graphite electrodes. Cytochrome P450scc was also deposited onto both gold nanoparticle-modified and bare rhodium–graphite electrodes, in order to have a comparison with our previous works in this field. Cyclic voltammetry indicated largest enhanced activity of the enzyme at the MWCNT-modified surface. The role of the nanotubes in mediating electron transfer to the cytochrome P450scc was verified as further improved with respect to the case of rhodium–graphite electrodes modified by the use of gold nanoparticles. The sensitivity of our system in cholesterol sensing is higher by orders of magnitude with respect to other similar systems very recently published that are based on cholesterol oxidase and esterase. The electron transfer improvement attained by the use of MWCNT in P450-based cholesterol biosensors was demonstrated to be larger than 2.4 times with respect to the use of gold nanoparticles and 17.8 times larger with respect to the case of simple bare electrodes. The sensitivity was equal to 1.12 μA/(mM mm 2) and the linearity of the biosensor response was improved with respect to the use of gold nanoparticles.
doi_str_mv 10.1016/j.bios.2008.03.008
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source MEDLINE; Elsevier ScienceDirect Journals Complete
subjects Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Cholesterol
Cholesterol - analysis
Cholesterol Side-Chain Cleavage Enzyme - chemistry
Cytochrome
Electrodes
Electron transfer
Multi-walled carbon nanotubes
Nanotubes, Carbon - chemistry
Screen-printed electrodes
Sensitivity and Specificity
title Screen-printed electrodes based on carbon nanotubes and cytochrome P450scc for highly sensitive cholesterol biosensors
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