Comparative Study of Polyphenol Scavenging Activities Assessed by Different Methods

The effect of procyanidin solutions on superoxide anion radicals was studied with an enzymatic method and their EC50 values were determined. A comparative study of the results suggested that the free radical scavenger potential of these phenolic compounds closely depends on their chemical and stereochemical structures. Oligomeric procyanidins were isolated in different fractions from grapes and wines by low- and high-pressure liquid chromatography. These compounds were found to be efficient free radical scavengers even for the weak concentrations in wines. Their activity in grapes or wines was much stronger than that of other commercially available natural antioxidants (such as ascorbic acid and gallic acid). The effect of tannins isolated from grapes on different radicals was analyzed according to three distinct methods: an enzymatic method for superoxide anion radicals (O2 •-), a chemical method for the stable 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•), and an immunochemical method to study the scavenging activity of seed procyanidins on DNA lesions induced by the radical HO•. Keywords: Grape seeds; wine; procyanidin; radical scavenger; DNA lesions