Synthesis and Binding Properties of Oligonucleotides Carrying Nuclear Localization Sequences

The synthesis of oligonucleotides carrying nuclear localization peptide sequences is described using two strategies:  first, oligonucleotides carrying a thiol group at the 5‘ end were reacted with maleimido peptides; second, peptide and oligonucleotide were prepared stepwise on the same support, yie...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bioconjugate chemistry 1999-11, Vol.10 (6), p.1005-1012
Hauptverfasser: de la Torre, Beatriz García, Albericio, Fernando, Saison-Behmoaras, Ester, Bachi, Angela, Eritja, Ramon
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The synthesis of oligonucleotides carrying nuclear localization peptide sequences is described using two strategies:  first, oligonucleotides carrying a thiol group at the 5‘ end were reacted with maleimido peptides; second, peptide and oligonucleotide were prepared stepwise on the same support, yielding oligonucleotide-3‘-peptide conjugates. This second approach was thoroughly studied. Using amino acids and small peptides as model compounds, some side reactions were analyzed, detected, and minimized. Oligonucleotides complementary to Ha-ras gene and carrying nuclear localization peptides at the 3‘ and 5‘ ends were prepared. Melting temperature studies showed that duplexes containing nuclear localization peptides were more stable than duplexes with unmodified oligonucleotides. Moreover, oligonucleotide−peptide conjugates maintain a good mismatch discrimination when they bind to their target RNA.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc990046l